Estimation of PMI depends on the changes in ATP and its degradation products
Estimation of PMI depends on the changes in ATP and its degradation products
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PMI的估算取决于ATP及其降解产物的变化
DOI：
10.1016/j.legalmed.2013.03.004
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发表时间：
2013-09-01
期刊：
影响因子：
1.5
通讯作者：
Wang, Zhen-Yuan
中科院分区：
文献类型：
--
作者：
Mao, Shiwei;Fu, Gaowen;Wang, Zhen-Yuan
估计死亡时间，或死后间隔（PMI），一直是现代法医学调查的最大困难之一。本研究探讨了大鼠多器官中三磷酸腺苷（ATP）分解产物浓度与PMI的相关性。在4 ℃或20 ℃的尸体中，在不同时间点收获大鼠的脑、脾和肾。使用高效液相色谱法（HPLC）定量与ATP降解相关的代谢物浓度。计算K值（Kv = 100 x（Hx + HxR）/（ATP + ADP + AMP + IMP + HxR + Hx）），并与每个器官的PMI和温度相关。结果表明，K值是一个强大的指数，PMI的基础上高度显着的线性相关性PMI和ATP分解产物的浓度估计。与现有的研究方法相比，ATP及其降解产物的变化趋势可能是法医实践中估计PMI的一种更好的方法。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Estimating the time since death, or postmortem interval (PMI), has been one of the biggest difficulties in modern forensic investigation. This study tests if the concentrations of breakdown products of adenosine triphosphate (ATP) correlate with PMI in multiple organs from rat. Brains, spleens, and kidneys of rats were harvested at different time points in carcasses maintained at 4 degrees C or 20 degrees C. High Performance Liquid Chromatography (HPLC) was used to quantify concentrations of metabolites related to ATP degradation. A K value (Kv = 100 x (Hx + HxR)/(ATP + ADP + AMP + IMP + HxR + Hx)) was calculated and correlated with PMI for each organ and temperature. The results indicate that the K value is a robust index for the estimation of PMI based on highly significant linear correlations between PMI and concentrations of ATP breakdown products. Compared with other current research methods, the changing tendency of ATP and its degradation products may be potentially a better way for the estimation of PMI in medico-legal practice. (C) 2013 Elsevier Ireland Ltd. All rights reserved.