Occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system, Paraná, Southern Brazil
Occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system, Paraná, Southern Brazil
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DOI：
10.1590/s1984-29612015051
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发表时间：
2015-09-01
期刊：
影响因子：
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通讯作者：
Freire, Roberta Lemos
中科院分区：
文献类型：
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作者：
Almeida, Jonatas Campos;Martins, Felippe Danyel Cardoso;Freire, Roberta Lemos
本研究的目的是调查隐孢子虫的发生。和贾第虫属类孢在公共水处理系统中。收集原水和处理水的样品，并使用膜过滤技术进行浓缩。对样品进行直接免疫荧光试验。使用商业试剂盒进行DNA提取，并将提取的DNA进行巢式PCR反应（n-PCR）和测序。免疫荧光检测2/24份（8.33%）水样阳性。在n-PCR和测序中，2/24（8.33%）的原水样品为贾第虫属阳性，隐孢子虫阳性率为8.33%（2/24）。测序结果显示隐孢子虫和贾第鞭毛虫DNA。原水中浊度、色度与隐孢子虫之间存在中等程度的相关性。浊度和贾第虫之间的关系。这些原生动物在水中的存在表明需要对水处理公司进行监测。
The purpose of this study was to investigate the occurrence of Cryptosporidium spp. and Giardia spp. in a public water-treatment system. Samples of raw and treated water were collected and concentrated using the membrane filtration technique. Direct Immunofluorescence Test was performed on the samples. DNA extraction using a commercial kit was performed and the DNA extracted was submitted to a nested-PCR reaction (n-PCR) and sequencing. In the immunofluorescence, 2/24 (8.33%) samples of raw water were positive for Giardia spp.. In n-PCR and sequencing, 2/24 (8.33%) samples of raw water were positive for Giardia spp., and 2/24 (8.33%) samples were positive for Cryptosporidium spp.. The sequencing showed Cryptosporidium parvum and Giardia duodenalis DNA. In raw water, there was moderate correlation among turbidity, color and Cryptosporidium spp. and between turbidity and Giardia spp.. The presence of these protozoans in the water indicates the need for monitoring for water-treatment companies.