Label-free antibody-antigen binding detection by optical sensor array based on surface-synthesized gold nanoparticles.
Label-free antibody-antigen binding detection by optical sensor array based on surface-synthesized gold nanoparticles.
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DOI：
10.1016/j.bios.2007.11.023
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发表时间：
2008-03
影响因子：
12.6
通讯作者：
R. Olkhov;A. M. Shaw
中科院分区：
文献类型：
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作者：
R. Olkhov;A. M. Shaw
从印刷种子颗粒在玻璃基板上原位生长的金纳米颗粒已被制造成生物传感器阵列。所得表面的光散射特性显示出对局部折射率变化的敏感性。每个阵列点用纤维蛋白原或牛血清白蛋白功能化，散射辐射用于监测全血抗血清中抗体与抗原无标记结合时的折射率变化。实时收集数据，并通过动力学分析得出特异性抗体-抗原结合的关联速率常数。最低抗体浓度检测灵敏度为100nM。
Gold nanoparticles grown in situ from printed seed particles on a glass substrate have been fabricated into a biosensor array. The light-scattering properties of the resulting surfaces show sensitivity to changes in the local refractive index. Each array spot is functionalized with fibrinogen or bovine serum albumin and scattered radiation is used to monitor the refractive index change on label-free binding of the antibodies to their antigens from whole blood antiserum. Data were collected real-time and the association rate constants for the specific antibody–antigen binding were derived from a kinetic analysis. The minimum antibody concentration detection sensitivity is of 100nM.