Lymphatic vessel density as a predictor of lymph node metastasis and its relationship with prognosis in urothelial carcinoma of the bladder
Lymphatic vessel density as a predictor of lymph node metastasis and its relationship with prognosis in urothelial carcinoma of the bladder
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DOI：
10.1111/j.1464-410x.2010.09725.x
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发表时间：
2011-06-01
影响因子：
4.5
通讯作者：
Qi, Lin
中科院分区：
文献类型：
--
作者：
Zhou, Mi;He, Leye;Qi, Lin
证据等级4关于该主题已知的信息？这项研究增加了什么？VEGF-C在某些肿瘤组织中表达上调。在这项研究中，我们发现VEGF-C在膀胱癌中的表达也增加。VEGF-C表达的增加可能对膀胱癌淋巴结转移有影响。为了确定淋巴管密度（LVD）与淋巴结转移（LNM）的相关性，研究血管内皮生长因子（VEGF）-C mRNA表达对LVD的影响，并评价LVD对膀胱尿路上皮癌（布克）预后的影响。用抗VEGF受体3（VEGFR-3）的多克隆抗体对淋巴管进行免疫染色。tissues.center采用逆转录聚合酶链reaction.center（RT-PCR）检测VEGF-C mRNA的表达水平，并分析VEGF-C mRNA表达水平与LVD的相关性。随着VEGF-C表达水平的增高，肿瘤内和瘤周LVD均显著增加同时，淋巴管侵犯和淋巴结转移的布克癌患者，瘤内和瘤周LVD也明显增加（P < 0.05）此外，瘤周LVD和LNM增加预示无复发生存率低结论：提示在布克癌中，VEGF-C表达可能与肿瘤lymphangiogenesis.center周围LVD和LNM高的患者倾向于具有较差的www.example.com dotprognosis.center。抑制阻断VEGF-C/VEGFR-3通路可能减弱淋巴管生成，并代表膀胱尿路上皮癌研究性治疗的新靶点。
Level of Evidence 4What's known on the subject? and What does the study add?VEGF-C has been found up-regulated in some kind of tumour tissues. In this study, we found that the expression of VEGF-C was also increased in bladder cancer. The increase of VEGF-C may have an influence on lymphatic node metastasis of bladder cancer.OBJECTIVEcenter dot To determine the correlation of lymphatic vessel density (LVD) with lymph node metastasis (LNM), investigate the impact of vascular endothelial growth factor (VEGF)-C mRNA expression on LVD, and evaluate the impact of LVD on prognosis of bladder urothelial carcinoma (BUC).PATIENTS AND METHODScenter dot In 56 samples of BUC, the lymphatic vessels were immunostained with polyclonal antibodies against VEGF receptor 3 (VEGFR-3). LVD was evaluated in both intratumoural and peritumoural tissues.center dot The expression level of VEGF-C mRNA was assessed by reverse transcriptase-polymerase chain reaction.center dot The correlation of LVD with VEGF-C mRNA and other clinicopathological parameters was also investigated.RESULTScenter dot VEGFR-3 was expressed in lymphatic vessel endothelial cytoplasm. As the expression level of VEGF-C became higher, the intratumoural and peritumoural LVD increased significantly (P < 0.05).center dot At the same time, increased intratumoural and peritumoural LVD also presented in patients with lymphatic vessel invasion and LNM of BUC (P < 0.05).center dot In addition, increased peritumoural LVD and LNM predicted a poor recurrence-free survival (P < 0.05).CONCLUSIONScenter dot It is suggested that in BUC, VEGF-C expression may contribute to lymphangiogenesis.center dot Patients with high peritumoural LVD and LNM tend to have a poor prognosis.center dot Inhibition of the blocking VEGF-C/VEGFR-3 pathway may attenuate lymphangiogenesis and represent a new target for investigational treatment of urothelial carcinoma of the bladder.