Chinese Cabbage BrCAP Has Potential Resistance against Plasmodiophora brassicae
Chinese Cabbage BrCAP Has Potential Resistance against Plasmodiophora brassicae
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DOI：
10.3390/horticulturae9040517
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发表时间：
2023-04
期刊：
影响因子：
3.1
通讯作者：
J. Zou;Shi-qi Gao;Bo Zhang;Wenjie Ge;Jing Zhang;R. Ji
中科院分区：
文献类型：
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作者：
J. Zou;Shi-qi Gao;Bo Zhang;Wenjie Ge;Jing Zhang;R. Ji
由根肿病菌（Plasmodiophora brassicae Woronin，P. brassicae）引起的根肿病严重影响了白菜类作物的生产，但P. brassicae与白菜类作物的互作机制尚不清楚。在前期的研究中，我们通过双向电泳和质谱分析，在接种和未接种芸苔疫霉的大白菜根中发现了一个差异表达蛋白G15，G15与Bra 011464相匹配。在本研究中，Bra 011464与拟南芥CAP有94%的同源性，命名为BrCAP。BrCAP在大白菜根中的表达量最高，茎和叶中的表达量最低，接种P. brassicae的大白菜根中BrCAP的表达量显著高于对照。通过实时定量聚合酶链反应（RT-qPCR）和原位杂交验证结果。亚细胞定位结果表明，BrCAP定位于叶表皮细胞的叶绿体上。为了验证BrCAP的功能，发现拟南芥突变体cap比野生型（WT）更易感染芸苔疫霉，表明BrCAP在大白菜对芸苔疫霉的抗性进程中具有潜在的作用。
Clubroot disease caused by Plasmodiophora brassicae Woronin (P. brassicae) has seriously influenced the production of Brassica rapa crops, but the interaction mechanism between P. brassicae and Brassica rapa is not clear. In our previous study, a differentially expressed protein, G15, was found between the roots of Chinese cabbage inoculated and un-inoculated with P. brassicae through two-dimensional electrophoresis (2-DE) and mass spectrometry, and G15 was matched with Bra011464. In this study, Bra011464 was found to have a 94% percent identity with Arabidopsis thaliana CAP, named BrCAP. The expression of BrCAP was the highest in the root compared with the stems and leaves of Chinese cabbage, and its expression in the roots of Chinese cabbage inoculated with P. brassicae was significantly higher than that in the control. The results were verified by real-time quantitative polymerase chain reaction (RT-qPCR) and in situ hybridization. Subcellular localization showed that BrCAP was localized on chloroplasts of leaf epidermal cells. To verify the function of BrCAP, it was found that the Arabidopsis thaliana mutant cap was more susceptible to infection with P. brassicae than the wild type (WT), which suggested that BrCAP has a potential role in the resistance progress of Chinese cabbage to P. brassicae.