Cartilage formation by cultured chondrocytes in a new scaffold made of poly(L-lactide-ε-caprolactone) sponge
Cartilage formation by cultured chondrocytes in a new scaffold made of poly(L-lactide-ε-caprolactone) sponge
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DOI：
10.1053/joms.2000.7262
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发表时间：
2000-07-01
影响因子：
1.9
通讯作者：
Kimata, K
中科院分区：
文献类型：
--
作者：
Honda, M;Yada, T;Kimata, K
目的：研究软骨细胞在体外培养后接种到新开发的可生物降解海绵中形成异位软骨组织的能力。材料与方法：从Lewis大鼠肋软骨中提取软骨细胞进行培养，使其增殖，然后将其接种于由可生物降解聚合物聚l -乳酸- ε -己内酯组成的海绵中。将软骨细胞和海绵的复合材料皮下移植到裸鼠体内，4周后取出进行组织学和Northern blot分析。结果：苏木精和伊红染色显示海绵内形成软骨样结构。对复合材料的总RNA进行Northern blot分析显示，存在约9 kb的聚集蛋白转录本。结论：聚l -乳酸- ε -己内酯海绵体系适合作为组织工程软骨的基质。(C) 2000美国口腔颌面外科医师协会。
Purpose: This study investigated the ability of chondrocytes grown in culture and inoculated into a newly developed biodegradable sponge to form ectopic cartilage tissue.Materials and Methods: Chondrocytes obtained from costochondral cartilage dissected from Lewis rats were cultured to allow proliferation and then were inoculated into a sponge consisting of a biodegradable polymer, poly (L-lactide-epsilon-caprolactone). The composites of chondrocytes and sponge were transplanted subcutaneously into Nude mice and removed after 4 weeks for histologic and Northern blot analysis.Results: Staining with hematoxylin and eosin showed the formation of a cartilage-like structure in the sponge. Northern blot analysis of the total RNA in the composites showed the presence of aggrecan transcripts of about 9 kb.Conclusion: The poly (L-lactide-epsilon-caprolactone) sponge system, is suitable as a matrix for tissue-engineered cartilage. (C) 2000 American Association of Oral and Maxillofacial Surgeons.