Electroactive Silk Fibroin Films for Electrochemically Enhanced Delivery of Drugs
Electroactive Silk Fibroin Films for Electrochemically Enhanced Delivery of Drugs
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DOI：
10.1002/mame.202000130
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发表时间：
2020-06
影响因子：
3.9
通讯作者：
Seyed T. Mousavi;G. Harper;S. Municoy;Mark D. Ashton;David Townsend;G. Alsharif;V. Oikonomou
中科院分区：
文献类型：
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作者：
Seyed T. Mousavi;G. Harper;S. Municoy;Mark D. Ashton;David Townsend;G. Alsharif;V. Oikonomou
能够控制药物输送的生物材料具有治疗多种疾病的潜力。本文描述了基于导电丝素膜的给药装置的制备。铸造家蚕丝素的水溶液，然后干燥和退火以赋予丝素β -薄片，确保材料在水中的进一步处理是稳定的；通过在丝素基质中产生吡罗和3 -氨基- 4 -羟基苯磺酸共聚物的互穿网络，丝素薄膜具有导电性(通过各种技术表征，包括圆二色性、傅里叶变换红外光谱、核磁共振、拉曼光谱、电阻测量、扫描电子显微镜-能量色散X射线光谱、热重分析、X射线衍射、和X射线光电子能谱)。成纤维细胞粘附在生物材料表面（使用（3‐（4,5‐二甲基噻唑‐2‐基）‐2,5‐二苯基溴化四唑）测定法评估活力，并使用共聚焦显微镜观察），荧光标记的药物（Texas‐Red庆大霉素）可以电化学加载并释放（µg cm−2的量），以响应电刺激的应用。
Biomaterials capable of controlling the delivery of drugs have the potential to treat a variety of conditions. Herein, the preparation of electrically conductive silk fibroin film‐based drug delivery devices is described. Casting aqueous solutions of Bombyx mori silk fibroin, followed by drying and annealing to impart β‐sheets to the silk fibroin, assure that the materials are stable for further processing in water; and the silk fibroin films are rendered conductive by generating an interpenetrating network of a copolymer of pyrrole and 3‐amino‐4‐hydroxybenzenesulfonic acid in the silk fibroin matrix (characterized by a variety of techniques including circular dichroism, Fourier‐transform infrared spectroscopy, nuclear magnetic resonance, Raman spectroscopy, resistance measurements, scanning electron microscopy‐energy dispersive X‐ray spectroscopy, thermogravimetric analysis, X‐ray diffraction, and X‐ray photoelectron spectroscopy). Fibroblasts adhere on the surface of the biomaterials (viability assessed using an (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) assay and visualized using a confocal microscope), and a fluorescently labeled drug (Texas‐Red Gentamicin) can be loaded electrochemically and released (µg cm−2 quantities) in response to the application of an electrical stimulus.