Establishment of a Novel In Vitro Test Setup for Electric and Magnetic Stimulation of Human Osteoblasts
Establishment of a Novel In Vitro Test Setup for Electric and Magnetic Stimulation of Human Osteoblasts
复制标题
DOI：
10.1007/s12013-014-9984-6
复制
发表时间：
2014-11-01
影响因子：
2.6
通讯作者：
Bader, R.
中科院分区：
文献类型：
--
作者：
Grunert, P. C.;Jonitz-Heincke, A.;Bader, R.
当发生大的缺损时，骨再生可以通过骨移植和生物物理刺激如电和磁刺激（EMS）来支持。临床上建立的EMS模式是外部线圈和外科植入物，如电感应螺钉系统，其结合了磁场和电场，例如。例如，在一个实施例中，用于治疗缺血性骨坏死或假关节。为了优化该植入系统，设计了体外试验装置，以研究EMS对不同3D支架（基于磷酸钙和胶原）上的人成骨细胞的影响。在电池实验之前，通过测量EMS引起的电参数进行了装置的数值模拟以及实验验证。将人成骨细胞（3 × 10（5）个细胞）接种到支架上并培养。24小时后，将螺钉植入物（斯特赖克ASNIS III s系列）置于支架中心，并施加EMS（每天3 x 45分钟，20 Hz）3天。随后测定细胞活力和1型胶原（Col 1）合成。数值模拟和验证表明，支架内的电场分布足够。电势的实验测量显示与模拟的偏差很小。细胞对刺激的反应随支架材料和刺激模式而变化。EMS刺激的细胞表现出代谢活性的显著降低，特别是在胶原支架上。相比之下，胶原蛋白和非烧结磷酸钙支架上的Col 1/代谢活性比在3天后显著增加。排他性的磁刺激表现出类似的，但不显着的趋势，代谢活动和Col 1的合成。细胞测试表明，新的测试设置是一个有价值的工具，在体外测试和参数优化的临床使用的电感应螺钉系统。它结合了磁刺激和电刺激，允许在体外研究其对人类成骨细胞的影响。
When large defects occur, bone regeneration can be supported by bone grafting and biophysical stimuli like electric and magnetic stimulation (EMS). Clinically established EMS modes are external coils and surgical implants like an electroinductive screw system, which combines a magnetic and electric field, e. g., for the treatment of avascular bone necrosis or pseudarthrosis. For optimization of this implant system, an in vitro test setup was designed to investigate effects of EMS on human osteoblasts on different 3D scaffolds (based on calcium phosphate and collagen). Prior to the cell experiments, numerical simulations of the setup, as well as experimental validation, via measurements of the electric parameters induced by EMS were conducted. Human osteoblasts (3 x 10(5) cells) were seeded onto the scaffolds and cultivated. After 24 h, screw implants (Stryker ASNIS III s-series) were centered in the scaffolds, and EMS was applied (3 x 45 min per day at 20 Hz) for 3 days. Cell viability and collagen type 1 (Col1) synthesis were determined subsequently. Numerical simulation and validation showed an adequate distribution of the electric field within the scaffolds. Experimental measurements of the electric potential revealed only minimal deviation from the simulation. Cell response to stimulation varied with scaffold material and mode of stimulation. EMS-stimulated cells exhibited a significant decrease of metabolic activity in particular on collagen scaffolds. In contrast, the Col1/metabolic activity ratio was significantly increased on collagen and non-sintered calcium phosphate scaffolds after 3 days. Exclusive magnetic stimulation showed similar but nonsignificant tendencies in metabolic activity and Col1 synthesis. The cell tests demonstrate that the new test setup is a valuable tool for in vitro testing and parameter optimization of the clinically used electroinductive screw system. It combines magnetic and electric stimulation, allowing in vitro investigations of its influence on human osteoblasts.