Lycorine induces apoptosis of bladder cancer T24 cells by inhibiting phospho-Akt and activating the intrinsic apoptotic cascade
Lycorine induces apoptosis of bladder cancer T24 cells by inhibiting phospho-Akt and activating the intrinsic apoptotic cascade
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DOI：
10.1016/j.bbrc.2016.12.168
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发表时间：
2017-01-29
影响因子：
3.1
通讯作者：
Huang, Peng
中科院分区：
文献类型：
--
作者：
Wang, Chongshan;Wang, Qiongren;Huang, Peng
石蒜碱是石蒜科石蒜属植物中提取的一种生物碱，对多种人实体瘤和白血病细胞具有抗肿瘤活性，并对多种细胞信号分子产生广泛影响。然而，石蒜碱对膀胱癌的作用尚未研究。在这项研究中，我们证明了石蒜碱诱导人膀胱癌T24细胞凋亡，这种作用是通过抑制磷酸化Akt表达和随后激活caspase-3和Bax介导的。在体内实验中，将T24细胞皮下植入nu/ nu小鼠的右后侧腹。石蒜碱治疗14天显着抑制肿瘤生长与对照组相比。总的来说，我们的研究结果表明石蒜碱抑制Akt通路，激活内在的凋亡级联反应，导致膀胱癌细胞的凋亡。我们认为石蒜碱可以成为膀胱癌患者的一种可行的治疗选择。(C)2016 Elsevier Inc. All rights reserved.
Lycorine, an alkaloid extracted from Amaryllidaceae genera, exhibits antitumor activities against several human solid-tumor and leukemia cells with extensive influence on various cell signaling molecules. However, the effect of lycorine on bladder cancer has not yet been investigated. In this study, we demonstrated that lycorine induced apoptosis in human bladder cancer T24 cells, an effect that is mediated via inhibition of phospho-Akt expression and the consequent activation of caspase-3 and Bax in vitro. In an in vivo experiment, T24 cells were subcutaneously implanted in the right rear flank of nu/ nu mice. Lycorine treatment for 14 days significantly inhibited tumor growth compared with that in controls. Collectively, our findings suggest that lycorine suppressed the Akt pathway and activated the intrinsic apoptotic cascade, leading to the apoptosis of bladder cancer cells. We suggest that lycorine can be a viable therapeutic option for bladder cancer patients. (C) 2016 Elsevier Inc. All rights reserved.