A chimeric human T-Cell lymphotropic virus type I with the envelope glycoprotein of Moloney murine leukemia virus is infectious for murine cells
A chimeric human T-Cell lymphotropic virus type I with the envelope glycoprotein of Moloney murine leukemia virus is infectious for murine cells
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DOI：
10.1128/jvi.76.15.7883-7889.2002
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发表时间：
2002-08-01
影响因子：
5.4
通讯作者：
Tangy, F
中科院分区：
文献类型：
--
作者：
Delebecque, F;Pramberger, K;Tangy, F
我们构建了一个嵌合的人类T细胞嗜淋巴细胞病毒1型（HTLV-1）的前病毒，其中原来的包膜前体序列被替换为亲嗜性莫洛尼鼠白血病病毒（Mo-MuLV）。通过瞬时转染该嵌合前病毒产生的嵌合颗粒对鼠细胞如NIH 3T3成纤维细胞、淋巴样EL4细胞和原代CD4（+）T淋巴细胞具有感染性，而HTLV-1颗粒则没有。当位于MuLV包膜糖蛋白羧基末端的R肽缺失时，嵌合颗粒的感染性增加了10倍。被DeltaR嵌合病毒感染的原代鼠CD4（+）T淋巴细胞释放出可将感染扩散到其他幼稚鼠淋巴细胞的颗粒。这种嵌合病毒，与Mo-MuLV包膜糖蛋白和HTLV-1的复制特性，应该是有用的，在研究HTLV-1的发病机制在小鼠模型。
We constructed a chimeric human T-cell lymphotropic virus type 1 (HTLV-1) provirus in which the original envelope precursor sequence was replaced by that of ecotropic Moloney murine leukemia virus (Mo-MuLV). Chimeric particles produced by transient transfection of this chimeric provirus were infectious for murine cells, such as NIH 3T3 fibroblasts, lymphoid EL4 cells, and primary CD4(+) T lymphocytes, whereas HTLV-1 particles were not. The infectivity of chimeric particles increased 10 times when the R peptide located at the carboxy terminus of the MuLV envelope glycoprotein was deleted. Primary murine CD4(+) T lymphocytes, infected by the DeltaR chimeric virus, released particles that could spread the infection to other naive murine lymphoid cells. This chimeric virus, with the Mo-MuLV envelope glycoprotein and the replication characteristics of HTLV-1, should be useful in studying the pathogenesis of HTLV-1 in a mouse model.