The Changes of Fourier Transform Infrared Spectrum in Rat Brain
The Changes of Fourier Transform Infrared Spectrum in Rat Brain
复制标题
大鼠脑内傅里叶变换红外光谱的变化
DOI：
10.1111/j.1556-4029.2011.02036.x
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发表时间：
2012-05-01
影响因子：
1.6
通讯作者：
Wang, Zhen-Yuan
中科院分区：
文献类型：
--
作者：
Ke, Yong;Li, Yang;Wang, Zhen-Yuan
死亡时间（postmortem interval，PMI）的估计是法医学中最困难的问题之一，目前有多种方法用于PMI的估计。研究大鼠死后0144 h脑组织衰减全反射-傅里叶变换红外光谱（ATR-FTIR）的变化。检查了主要吸收带的强度比（I1066/I1392、I1168/I1392、I1234/I1454、I1301/I1392、I1647/I2956、I2921/I2850和I1647/I1539）。随着PMI的增加，大鼠脑组织的光谱发生了明显的变化。2871和1737/cm处的条带随时间的延长逐渐减弱，至96和72 h时消失。相对吸收强度与PMI呈线性相关，其中I1234/I1454的相关性较强（r = 0.973）。我们的研究结果表明，ATR-FTIR光谱可能是一个有用的技术估计PMI。
Estimation of the time since death (postmortem interval [PMI]) is one of the most difficult problems in forensic investigations, and many methods currently are utilized to estimate the PMI. The goal of this study was to investigate the changes of attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectra of rat brain from postmortem time 0144 h. The intensity ratios of major absorbance bands were examined (I1066/I1392, I1168/I1392, I1234/I1454, I1301/I1392, I1647/I2956, I2921/I2850, and I1647/I1539). The spectra of rat brain displayed prominent changes with increasing PMI. The band at 2871 and 1737 per cm became weak with the time increasing and even disappeared at postmortem 96 and 72 h, respectively. A close linear correlation was shown between the relative absorption intensity and the PMI, and the I1234/I1454 offered a stronger correlation (r = 0.973). Our results indicate that ATR-FTIR spectroscopy may be a useful technique for estimating the PMI.