Different isoforms of the C. elegans FGF receptor are required for attraction and repulsion of the migrating sex myoblasts.
Different isoforms of the C. elegans FGF receptor are required for attraction and repulsion of the migrating sex myoblasts.
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吸引和排斥迁移的性成肌细胞需要不同亚型的线虫 FGF 受体。
DOI：
10.1016/j.ydbio.2008.03.026
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发表时间：
2008
影响因子：
2.7
通讯作者：
Stern,MichaelJ
中科院分区：
文献类型：
--
作者：
Lo,Te-Wen;Branda,CatherineS;Huang,Peng;Sasson,IsaacE;Goodman,SJay;Stern,MichaelJ
秀丽线虫的成纤维细胞生长因子受体EGL-15被选择性剪接，产生两种不同的主要亚型，它们的胞外结构域不同。EGL-15(5A)亚型对于移行性成肌细胞(SMS)的性腺化学吸引是必需的，而EGL-15(5B)亚型是生存所必需的。在这里，我们发现5A主要在M系中表达，而5B主要在皮下组织中表达，而M系导致了迁移的SM及其性肌肉后代。然而，组织特异性表达只解释了这两种受体亚型之间功能差异的一部分。5A在皮下表达时可实现5B的互惠功能，而5B不能进行SM趋化。因此，我们的数据表明，这两种异构体的结构差异导致了它们的功能差异。两条证据表明，5B亚型也在SM的迁移中发挥作用，这意味着当化学吸引被削弱时，它与观察到的排斥有关。因此，这两种异构体胞外区的结构差异可以决定性腺的吸引力或排斥性。
The Caenorhabditis elegans FGF receptor, EGL-15, is alternatively-spliced to yield two major isoforms that differ in their extracellular domains. The EGL-15(5A) isoform is necessary for the gonadal chemoattraction of the migrating sex myoblasts (SMs), while the EGL-15(5B) isoform is required for viability. Here we show that 5A is predominantly expressed in the M lineage, which gives rise to the migrating SMs and their sex muscle descendants, while 5B is predominantly expressed in the hypodermis. Tissue-specific expression, however, explains only part of the functional differences between these two receptor isoforms. 5A can carry out the reciprocal essential function of 5B when expressed in the hypodermis, but 5B is incapable of carrying out SM chemoattraction. Our data, therefore, indicate that the structural differences in these two isoforms contribute to their functional differences. Two lines of evidence indicate that the 5B isoform also plays a role in SM migration, implicating it in the repulsion that is observed when the chemoattraction is compromised. Thus, structural differences in the extracellular domains of these two isoforms can specify either attraction to or repulsion from the gonad.