Genome sequence and analysis of a broad-host range lytic bacteriophage that infects the Bacillus cereus group.
Genome sequence and analysis of a broad-host range lytic bacteriophage that infects the Bacillus cereus group.
复制标题
DOI：
10.1186/1743-422x-10-48
复制
发表时间：
2013-02-07
期刊：
影响因子：
4.8
通讯作者：
Kropinski AM
中科院分区：
文献类型：
--
作者：
El-Arabi TF;Griffiths MW;She YM;Villegas A;Lingohr EJ;Kropinski AM
关于感染低 G+C 含量的厚壁菌门革兰氏阳性宿主细菌的肌病毒科成员的信息相对较少。虽然迄今为止已分离出许多芽孢杆菌噬菌体，但只有极少数蜡状芽孢杆菌噬菌体得到了详细表征。在这里，我们展示了大型、有毒、宿主范围广泛的蜡状芽孢杆菌噬菌体 vB_BceM_Bc431v3 (Bc431v3) 的数据。 Bc431v3 具有 158,618 bp dsDNA 基因组，包含 239 个假定的开放阅读框 (ORF) 和编码 17 种不同氨基酸的 20 个 tRNA 基因。由于脉冲场凝胶电泳表明该噬菌体的基因组质量为 155-158 kb，因此 Bc431v3 DNA 似乎不包含芽孢杆菌噬菌体 SPO1 基因组中发现的长末端重复序列。 Bc431v3 在蛋白质水平上与蜡样芽孢杆菌噬菌体 BCP78、李斯特菌噬菌体 A511 和肠球菌噬菌体 ØEF24C 以及感染厚壁菌门的其他形态相关噬菌体（例如葡萄球菌噬菌体 K 和乳杆菌噬菌体 LP65）显示出显着的序列相似性。基于这些数据，我们建议将 Bc431v3 纳入 Spounavirinae 的成员；然而，由于最近处理了所有不同的分类信息，因此很难确定属。 Bc431v3 噬菌体含有一些非常不寻常的基因，例如编码假定的 tRNAHis 鸟苷基转移酶的 gp143。此外，它还携带一些似乎与宿主孢子形成调节因子相关的基因。它们是： gp098，编码与 FstK/SpoIIIE DNA 转运蛋白相关的推定分离蛋白； gp105，一种假定的分离蛋白； gp108，RNA聚合酶西格玛因子F/B；和，编码RNA聚合酶σ因子G的gp109。
Comparatively little information is available on members of the Myoviridae infecting low G+C content, Gram-positive host bacteria of the family Firmicutes. While numerous Bacillus phages have been isolated up till now only very few Bacillus cereus phages have been characterized in detail. Here we present data on the large, virulent, broad-host-range B. cereus phage vB_BceM_Bc431v3 (Bc431v3). Bc431v3 features a 158,618 bp dsDNA genome, encompassing 239 putative open reading frames (ORFs) and, 20 tRNA genes encoding 17 different amino acids. Since pulsed-field gel electrophoresis indicated that the genome of this phage has a mass of 155-158 kb Bc431v3 DNA appears not to contain long terminal repeats that are found in the genome of Bacillus phage SPO1. Bc431v3 displays significant sequence similarity, at the protein level, to B. cereus phage BCP78, Listeria phage A511 and Enterococcus phage ØEF24C and other morphologically related phages infecting Firmicutes such as Staphylococcus phage K and Lactobacillus phage LP65. Based on these data we suggest that Bc431v3 should be included as a member of the Spounavirinae; however, because of all the diverse taxonomical information has been addressed recently, it is difficult to determine the genus. The Bc431v3 phage contains some highly unusual genes such as gp143 encoding putative tRNAHis guanylyltransferase. In addition, it carries some genes that appear to be related to the host sporulation regulators. These are: gp098, which encodes a putative segregation protein related to FstK/SpoIIIE DNA transporters; gp105, a putative segregation protein; gp108, RNA polymerase sigma factor F/B; and, gp109 encoding RNA polymerase sigma factor G.