Diagnosis of arthritis using near-infrared fluorochrome Cy5.5
Diagnosis of arthritis using near-infrared fluorochrome Cy5.5
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DOI：
10.1097/01.rli.0000139008.04288.fd
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发表时间：
2004-10-01
影响因子：
6.7
通讯作者：
Kaiser, WA
中科院分区：
文献类型：
--
作者：
Hansch, A;Frey, O;Kaiser, WA
目的：近红外距离荧光(NIRF)成像是诊断活体生物过程的一种潜在工具。本研究旨在明确荧光染料Cy5.5在小鼠抗原性关节炎(AIA)中的诊断价值。材料和方法：在AIA的第7天(n=9只小鼠)，静脉注射Cy5.5前后(直至72小时)，测量炎症关节和对侧膝关节(后者为内对照)的荧光强度。激光共聚焦扫描显微镜观察Cy5.5关节沉积情况。用流式细胞仪检测培养的巨噬细胞(PMJ2-R)对染料的吞噬作用。用NIRF扫描和凝胶电泳法检测Cy5.5与血清蛋白的结合。结果：在2~72小时内，关节炎膝关节的荧光强度明显高于对侧关节。显微镜检查证实Cy5.5在滑膜中沉积。培养的巨噬细胞能主动吞噬Cy5.5。结论：Cy5.5的NIRF成像可以在体内识别关节炎关节，可能是由于非特异性沉积所致。
Purpose: Near-infrared range fluorescence (NIRF) imaging is a potential tool to diagnose biologic processes in vivo. This applicability study sought to define whether imaging with fluorochrome Cy5.5 can identify arthritis in murine antigen-induced arthritis (AIA).Materials and Methods: On day 7 of AIA (n = 9 mice), fluorescence intensities in inflamed and contralateral knee joints (the latter as internal control) were measured before and after intravenous injection of Cy5.5 (until 72 hours). Cy5.5 joint deposition was verified by confocal laser-scanning microscopy. Dye phagocytosis was evaluated in cultured macrophages (cell line PMJ2-R) by FACS analysis. Cy5.5 binding to serum protein was tested by NIRF scanning and gel electrophoresis.Results: Between 2 and 72 hours, the arthritic knee joints showed significantly higher fluorescence intensities compared with contralateral joints. Microscopy confirmed Cy5.5 deposition in the synovial membrane. Cultured macrophages actively phagocytosed Cy5.5. Cy5.5 bound mainly to albumin as the main serum protein.Conclusion: NIRF imaging with Cy5.5 can identify arthritic joints in vivo, likely due to nonspecific deposition.