Preparation and Dynamic Viscoelastic Characterization of Pepsin-Solubilized Collagen from Shark Skin Compared with Pig Skin
Preparation and Dynamic Viscoelastic Characterization of Pepsin-Solubilized Collagen from Shark Skin Compared with Pig Skin
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DOI：
10.2508/chikusan.70.227
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发表时间：
1999-07
期刊：
影响因子：
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通讯作者：
K. Yoshimura;Y. Chonan;K. Shirai
中科院分区：
文献类型：
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作者：
K. Yoshimura;Y. Chonan;K. Shirai
为了优化胃蛋白酶溶解胶原蛋白的制备工艺并考察其性质，对鲨鱼皮胶原蛋白和猪皮胶原蛋白进行了比较研究。与PC相比，SC不仅更易溶于乙酸，而且更易受胃蛋白酶的攻击。SC在20°C时的溶解度几乎达到100%，而PC溶解约50%。SC具有少量的胶原相关肽，其在20°C下用胃蛋白酶从SC中瞬时释放并最终消失。它们由非胶原和胶原来源组成。为了制备胃蛋白酶溶解的SC（PSSC），理想的是在20°C下进行胃蛋白酶处理超过48小时。胃蛋白酶增溶PC（PSPC）主要由α链组成，而PSSC主要由β链组成。在一定温度下，胶原溶液的弹性模量G'和粘性模量G”突然下降，同时tan δ迅速增加，反映了胶原的典型变性过程。PSSC和PSPC的G”小于酸溶性胶原（ASC），tan δ也大于ASC。结果表明，用胃蛋白酶去除端肽后，分子间相互作用减弱。认为动态粘弹性研究可以区分PSSC和PSPC，从而为评价胶原材料提供了一种有效的手段。
Aiming at optimizing the preparation of pepsin-solubilized collagen and investigating its properties, comparison between shark and pig skin collagens (SC and PC) was done. SC is not only more soluble in acetic acid but also more susceptible to pepsin-attack compared with PC. Solubilization of SC attained almost 100% at 20°C, while PC dissolved about 50%. SC has a small amount of collagen-associated peptides, which appeared transiently released from SC with pepsin at 20°C and disappeared ultimately. They are consisted of non-collagen and collagen origins. For preparation of pepsin-solubilized SC (PSSC), it is desirable to conduct the pepsin treatment at 20°C for more than 48 hours. Pepsin-solubilized PC (PSPC) mainly consisted of α chain, while PSSC mainly consisted of β chain. Elastic modulus G' and viscous modulus G" of collagen solution decreased suddenly with concomitant rapid increase in tan δ at a defined temperature, reflecting the typical denaturation process of collagen. G" of PSSC and PSPC was smaller than that of acid soluble collagen (ASC), and tan δ of PSSC and PSPC was also higher than that of ASC. It is suggested that the decrease of intermolecular interaction by removing telopeptide with pepsin. It is considered that the present dynamic viscoelasticity study could distinguish the difference between PSSC and PSPC, and hence provides an effective means of evaluating collagen materials.