Protein resistance of dextran and dextran-poly(ethylene glycol) copolymer films.
Protein resistance of dextran and dextran-poly(ethylene glycol) copolymer films.
复制标题
葡聚糖和葡聚糖-聚乙二醇共聚物膜的蛋白质抗性。
DOI：
10.1080/08927014.2011.584618
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发表时间：
2011
期刊：
影响因子：
2.7
通讯作者：
Trau,Matt
中科院分区：
文献类型：
--
作者：
Kozak,Darby;Chen,Annie;Bax,Jacinda;Trau,Matt
以有机硅颗粒为载体，研究了葡聚糖和葡聚糖-聚乙二醇共聚物膜的蛋白质抗性。以有机硅颗粒为固体合成载体，采用两步法制备了梳状支化葡聚糖-聚乙二醇共聚物膜。研究发现，与葡聚糖-−共聚物和先前研究的聚乙二醇膜相比，用3种相对分子质量(10,000，66,900和400,000 g−-1)的葡聚糖(10,000，66,900和400,000g摩尔聚乙二醇1)增加(0.1-1.2mgmPEG2)修饰的颗粒形成的抗蛋白质膜相对较差。研究发现，防污聚合物膜的防污效果与接枝量及其组成有关，其中聚乙二醇层的防污效果最好，其次是葡聚糖-聚乙二醇共聚物，而单用葡聚糖的防污效果最差。随着接枝葡聚糖量的增加，免疫球蛋白γ的吸附从∼5下降到0.5 mg m−2，但牛血清白蛋白的吸附在单层覆盖率(∼2 mg m−2)以上增加，表明较小的蛋白质在葡聚糖层内被三元吸附。
The protein resistance of dextran and dextran-poly(ethylene glycol) (PEG) copolymer films was examined on an organosilica particle-based assay support. Comb-branched dextran-PEG copolymer films were synthesized in a two step process using the organosilica particle as a solid synthetic support. Particles modified with increasing amounts (0.1–1.2 mg m−2) of three molecular weights (10,000, 66,900, 400,000 g mol−1) of dextran were found to form relatively poor protein-resistant films compared to dextran-PEG copolymers and previously studied PEG films. The efficacy of the antifouling polymer films was found to be dependent on the grafted amount and its composition, with PEG layers being the most efficient, followed by dextran-PEG copolymers, and dextran alone being the least efficient. Immunoglobulin gamma (IgG) adsorption decreased from ∼5 to 0.5 mg m−2with increasing amounts of grafted dextran, but bovine serum albumin (BSA) adsorption increased above monolayer coverage (∼2 mg m−2) indicating ternary adsorption of the smaller protein within the dextran layer.