Genetic and biochemical analysis of cis regulatory elements within the keratinocyte enhancer region of the human papillomavirus type 31 upstream regulatory region during different stages of the viral life cycle.
Genetic and biochemical analysis of cis regulatory elements within the keratinocyte enhancer region of the human papillomavirus type 31 upstream regulatory region during different stages of the viral life cycle.
复制标题
在病毒生命周期的不同阶段，对人乳头瘤病毒 31 型上游调节区的角质形成细胞增强子区域内的顺式调节元件进行遗传和生化分析。
DOI：
10.1128/jvi.78.2.612-629.2004
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发表时间：
2004
影响因子：
5.4
通讯作者：
Meyers,Craig
中科院分区：
文献类型：
--
作者：
Sen,Ellora;Alam,Samina;Meyers,Craig
Using linker scanning mutational analysis, we recently identified potentialcisregulatory elements contained within the 5′ upstream regulatory region (URR) domain and auxiliary enhancer (AE) region of the human papillomavirus type 31 (HPV31) URR involved in the regulation of E6/E7 promoter activity at different stages of the viral life cycle. For the present study, we extended the linker scanning mutational analysis to identify potentialciselements located in the keratinocyte enhancer (KE) region (nucleotides 7511 to 7762) of the HPV31 URR and to characterize cellular factors that bind to these elements under conditions representing different stages of the viral life cycle. The linker scanning mutational analysis identified viralciselements located in the KE region that regulate transcription in the presence and absence of any viral gene products or viral DNA replication and determine the role of host tissue differentiation on viral transcriptional regulation. Using electrophoretic mobility shift assays, we illustrated defined reorganization in the composition of cellular transcription factors binding to the samecisregulatory elements at different stages of the HPV differentiation-dependent life cycle. Our studies provide an extensive map of functional elements in the KE region of the HPV31 URR, identifycisregulatory elements that exhibit significant transcription regulatory potential, and illustrate changes in specific protein-DNA interactions at different stages of the viral life cycle. The variable recruitment of transcription factors to the sameciselement under different cellular conditions may represent a mechanism underlying the tight link between keratinocyte differentiation and E6/E7 expression.
