EVIDENCE FOR A COMMON MOLECULAR-ORIGIN OF THE CAPSULE GENE LOCI IN GRAM-NEGATIVE BACTERIA EXPRESSING GROUP-II CAPSULAR POLYSACCHARIDES
EVIDENCE FOR A COMMON MOLECULAR-ORIGIN OF THE CAPSULE GENE LOCI IN GRAM-NEGATIVE BACTERIA EXPRESSING GROUP-II CAPSULAR POLYSACCHARIDES
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DOI：
10.1111/j.1365-2958.1991.tb01899.x
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发表时间：
1991-05-01
影响因子：
3.6
通讯作者：
WEISGERBER, C
中科院分区：
文献类型：
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作者：
FROSCH, M;EDWARDS, U;WEISGERBER, C
革兰氏阴性菌的荚膜多糖在很大程度上有助于这些生物体的致病性。我们在这里表明，脑膜炎奈瑟氏球菌的不同血清群的荚膜基因位点的分子组织是类似的流感嗜血杆菌和大肠杆菌。在所有这些生物体中，参与将荚膜多糖转运到细胞表面的基因的高度同源性表明了包囊机制的共同分子起源。参与荚膜多糖转运的蛋白质符合ABC（ATP结合盒）转运蛋白的特征。通过对N.脑膜炎B型和E. coli K1，其荚膜由α-2，8-连接的多聚神经氨酸组成，观察到显著程度的同源性，表明荚膜多糖类型本身在这两种病原体中具有相同的进化起源。
Capsular polysaccharides of Gram-negative bacteria contribute to a large extent to the pathogenicity of these organisms. We show here that the molecular organization of the capsule gene loci in different serogroups of Neisseria meningitidis is similar to that of Haemophilus influenzae and Escherichia coli. A common molecular origin of the mechanisms of encapsulation is indicated by strong homology of the genes involved in transport of the capsular polysaccharides to the cell surface in all these organisms. The proteins involved in capsular polysaccharide transport fit the characteristics of ABC (ATP-binding cassette) transporters. Furthermore, by sequence comparison of the sialyltransferases of N. meningitidis B and E. coli K1, the capsule of which is composed of alpha-2,8-linked polyneuraminic acid, a significant degree of homology was observed, indicating that the capsular polysaccharide type itself has the same evolutionary origin in these two pathogens.