Molecular oscillation of Per1 and Per2 genes in the rodent brain: an in situ hybridization and molecular biological study.
Molecular oscillation of Per1 and Per2 genes in the rodent brain: an in situ hybridization and molecular biological study.
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啮齿动物大脑中 Per1 和 Per2 基因的分子振荡：原位杂交和分子生物学研究。
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
H. Okamura
中科院分区：
文献类型：
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作者：
D. Matsui;Seiichi Takekida;H. Okamura
昼夜节律最初是由一个基于转录翻译的振荡环产生的，其中PER1和PER2基因位于其中心。在大鼠脑内，PER1和PER2不仅在下丘脑视交叉上核(SCN)的神经元中有节律性表达，而且在包括大脑皮层在内的非SCN区域也有节律性表达。可能调节PER1和PER2基因转录的E-box增强子元件在大鼠和小鼠中高度保守。当E-box激活转录因子Clock和BMAL1共表达时，两种蛋白均呈现两种分子形式。这些高分子量形式的存在似乎与E-box介导的转录激活有关。这一机制可能不参与PER2介导的E-box抑制，因为加入PER2不会改变Clock和BMAL1的高分子形式的含量。
The circadian rhythm is originally generated by a transcription-translation based oscillatory loop where Per1 and Per2 genes locate in its central. In the rat brain, rhythmic expressions of Per1 and Per2 were observed not only in neurons of the hypothalamic suprachiasmatic nucleus (SCN) but also in those of non-SCN regions including the cerebral cortex. The E-box enhancer elements possible to regulate transcription of Per1 and Per2 genes were highly conserved in rats and mice. When E-box-activating transcription factors, CLOCK and BMAL1, were coexpressed, each of both proteins showed two molecular forms. The presence of these higher molecular weight forms seems to be correlated with the E-box mediated transcription activation. This mechanism might not be involved in the PER2 mediated suppression of E-box, since adding PER2 did not change the content of the higher molecular forms of CLOCK and BMAL1.
影响因子：
10.5
作者：
Kondratov, RV;Chernov, MV;Antoch, MP
通讯作者：
Antoch, MP