Comparative functional characterization of the feline foamy virus transactivator reveals its species specificity
Comparative functional characterization of the feline foamy virus transactivator reveals its species specificity
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DOI：
10.1016/j.virol.2003.09.039
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发表时间：
2004-01-05
期刊：
影响因子：
3.7
通讯作者：
Flügel, RM
中科院分区：
文献类型：
--
作者：
Bodem, J;Kang, YB;Flügel, RM
泡沫病毒（FV） Bel 1/Tas转录激活因子作为基因表达的关键调控因子，直接结合DNA Bel 1应答元件（BREs）在内部（IP）和5'LTR启动子中。本文报道了非同源猫泡沫病毒（FFV） BREs在两个启动子中的定位和病毒种特异性。结果表明，FFV Bel 1不与灵长类动物FV IP结合。灵长类动物FV Bell不能结合FFV IP.BRE。此外，我们发现FFV Bell的c端激活域与DNA结合无关，因为c端反显性阴性FFV Bell突变体仍然能够结合两个启动子。(C) 2003 Elsevier Inc.版权所有。
Foamy virus (FV) Bel 1/Tas transactivators act as key regulators of gene expression and directly bind DNA Bel 1 response elements (BREs) in both the internal (IP) and 5'LTR promoters. Here, we report the mapping and the virus species specificity of the nonhomologous feline foamy virus (FFV) BREs in both promoters. The data indicate that FFV Bel 1 did not bind the primate FV IP.BRE and that primate FV Bell was not capable of binding the FFV IP.BRE. In addition, we show that the C-terminal activation domain of FFV Bell does not contribute to DNA binding because a C-terininal trans-dominant negative FFV Bell mutant was still able to bind to both promoters. (C) 2003 Elsevier Inc. All rights reserved.