The outer surface proteins of Lyme disease borrelia spirochetes stimulate T cells to secrete interferon‐gamma (IFN‐γ): diagnostic and pathogenic implications
The outer surface proteins of Lyme disease borrelia spirochetes stimulate T cells to secrete interferon‐gamma (IFN‐γ): diagnostic and pathogenic implications
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莱姆病疏螺旋体螺旋体的外表面蛋白刺激 T 细胞分泌干扰素-γ (IFN-γ)：诊断和致病意义
DOI：
10.1111/j.1365-2249.1995.tb03134.x
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发表时间：
1995
影响因子：
4.6
通讯作者：
S. Bergström
中科院分区：
文献类型：
--
作者：
P. Forsberg;J. Ernerudh;C. Ekerfelt;M. Roberg;M. Vrethem;S. Bergström
莱姆病感染的晚期可能难以诊断，因为症状不具体，实验室检测不可靠，太不具体或不敏感。因此，通过使用灵敏的ELISPOT T细胞检测来评估这些患者的T细胞免疫反应，该检测可检测IFN - γ的分泌，即单细胞水平上的T辅助1 （Th1）反应。采用莱姆病阿氏疏螺旋体的三个亚细胞部分进行抗原刺激。外表面蛋白（Osp）部分引起了最强的反应，区分了伯氏疏疏体感染（n= 15）与其他神经系统疾病（n= 10）和正常对照（n= 12） （P= 0.0001）。然而，在一些对照中，更异质的超声伯氏体片段也引起了强烈的反应。鞭毛蛋白部分没有类似的T细胞刺激作用。当观察伯氏疏螺旋体感染亚群时，与其他伯氏疏螺旋体表现（n= 8）相比，中枢神经系统（CNS）感染（n= 7）显示血液中T细胞反应较低（P= 0.0128）。脑脊液（CSF）淋巴细胞来自3例中枢神经系统疏螺旋体病患者，与血液相比，脑脊液（CSF）中对Osp部分的反应更高，并且在2例没有任何鞘内特异性抗体合成的患者中也是如此。ELISPOT方法可用于检测博氏疏螺旋体感染中特异性IFN‐γ T细胞的反应。这种Th1反应很可能与致病相关。
Late stages of borrelia Lyme disease infections may be difficult to diagnose because of unspecific symptoms and unreliable laboratory tests, being too unspecific or insensitive. The T cell immune response was thus evaluated in these patients by using a sensitive ELISPOT T cell assay that detects the secretion of IFN‐γ, i.e. a T helper 1 (Th1) response on the single‐cell level. Three subcellular fractions of the Lyme borreliosis strain Borrelia afzelii were used for antigenic stimulation. The outer surface protein (Osp) fraction elicited the strongest response, discriminating between borrelia infections (n= 15) compared with other neurological diseases (n= 10) and normal controls (n= 12) (P= 0.0001). The more heterogeneous sonicated borrelia fraction also elicited a strong response, however, also in some of the controls. The flagellin fraction did not have a similar T cell‐stimulating effect. When looking at subgroups of borrelia infections, central nervous system (CNS) infections (n= 7) revealed a lower T cell response in blood (P= 0.0128) compared with other borrelia manifestations (n= 8). Cerebrospinal fluid (CSF) lymphocytes were available from three patients with CNS borreliosis, and all showed a compartmentalization with higher responses to the Osp fraction in CSF compared with blood, also in the two patients without any intrathecal‐specific antibody synthesis. The ELISPOT method is feasible for detecting a specific IFN‐γ T cell response in borrelia infections. This Th1 response may well be of pathogenic relevance.
莱姆病中伯氏疏螺旋体的淋巴细胞增殖反应。
影响因子：
39.2
作者：
Zoschke,DC;Skemp,AA;Defosse,DL
通讯作者：
Defosse,DL