Roles of intrinsic angiogenesis inhibitor, vasohibin, in cervical carcinomas
Roles of intrinsic angiogenesis inhibitor, vasohibin, in cervical carcinomas
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DOI：
10.1111/j.1349-7006.2010.01812.x
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发表时间：
2011-02-01
期刊：
影响因子：
5.7
通讯作者：
Sato, Yasufumi
中科院分区：
文献类型：
--
作者：
Yoshinaga, Kousuke;Ito, Kiyoshi;Sato, Yasufumi
本研究的目的是阐明vasohibin在宫颈癌中的关键作用。采用免疫组化方法研究血管内皮生长因子（VEGF）受体-2在内皮和微血管密度、淋巴管密度（LVD）中的表达比例。宫颈鳞癌61例，粘液腺癌18例，原位癌38例，正常宫颈上皮35例。我们研究了血管hibin的表达，并将其与VEGF受体-2 （VEGFR-2, KDR/flk-1）和CD34在间质内皮中的表达进行了比较。采用组织学评分(H)计算VEGF表达。用D2-40作为淋巴内皮细胞的标志物来观察LVD。正常宫颈上皮微血管密度显著低于CIS、SCC和腺癌（P < 0.05）。vasohibin在正常宫颈上皮组织中的表达率明显低于SCC和腺癌组织（P < 0.05）。正常宫颈上皮组织中VEGFR-2的表达率明显低于SCC和腺癌组织（P < 0.05）。正常宫颈上皮LVD明显低于CIS、SCC和腺癌（P < 0.05）。在正常宫颈上皮、CIS和SCC中，vasohibin的表达百分比与VEGFR-2的表达百分比呈正相关（P < 0.05, r(2) = 0.3018）。本研究首次阐明了人宫颈癌间质内皮细胞vasohibin表达与VEGFR-2表达之间的相关性。（癌症科学2011；102:446-451）
The aim of the present study is to clarify the critical roles of vasohibin in cervical carcinomas. We investigated the expression ratios of vasohibin and vascular endothelial growth factor (VEGF) receptor-2 on endothelium and microvessel density, lymphatic vessel density (LVD) by immunohistochemistry. Sixty-one squamous cell carcinoma (SCC), 18 mucinous adenocarcinoma (Adenocarcinoma), 38 carcinoma in situ (CIS), and 35 normal cervical epithelium were collected. We investigated the expression of vasohibin and compared it with the expression of VEGF receptor-2 (VEGFR-2, KDR/flk-1), and CD34 in the stromal endothelium. Expression of VEGF was counted using the histological score (H score). D2-40 was used as a marker for lymphatic endothelial cells to investigate LVD. The microvessel density of the normal cervical epithelium was significantly lower than that of CIS, SCC, and Adenocarcinoma (P < 0.05). The expression ratio of vasohibin in the normal cervical epithelium was significantly lower than that of SCC and Adenocarcinoma (P < 0.05). The expression ratio of VEGFR-2 of the normal cervical epithelium was significantly lower than that of SCC and Adenocarcinoma (P < 0.05). The LVD of the normal cervical epithelium was significantly lower than that of CIS, SCC, and Adenocarcinoma (P < 0.05). For normal cervical epithelium, CIS, and SCC, there was a moderate correlation between the expression percentage of vasohibin and the expression percentage of VEGFR-2 (P < 0.05, r(2) = 0.3018). This is the first study to elucidate the correlation between the expression of vasohibin in the stromal endothelial cells and the expression of VEGFR-2 in human cervical carcinomas. (Cancer Sci 2011; 102: 446-451)