The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain
The Arabidopsis gene CAD1 controls programmed cell death in the plant immune system and encodes a protein containing a MACPF domain
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DOI：
10.1093/pcp/pci095
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发表时间：
2005-06-01
影响因子：
4.9
通讯作者：
Yamaguchi, J
中科院分区：
文献类型：
--
作者：
Morita-Yamamuro, C;Tsutsui, T;Yamaguchi, J
为了澄清植物免疫过程中所涉及的，我们已经分离和表征了一个单一的隐性拟南芥突变体，cad 1（组成型激活细胞死亡1），它显示了一个表型，模仿的病变中看到的过敏反应（HR）。该突变体显示出发病机制相关（PR）基因的自发激活表达，并导致水杨酸（SA）增加32倍。用番茄假单胞菌致病变种DC3000接种cad 1突变体植物表明，cad 1突变导致细菌生长受到限制。CAD1的克隆揭示了该基因编码的蛋白质含有与动物先天免疫中涉及的补体成分和穿孔素蛋白的MACPF（膜攻击复合物和穿孔素）结构域具有显著同源性的结构域。此外，细胞死亡被抑制在转基因cad 1植物表达nahG，它编码的SA降解酶。因此，我们得出结论，CAD1蛋白负控制SA介导的植物免疫细胞程序性死亡途径。
To clarify the processes involved in plant immunity, we have isolated and characterized a single recessive Arabidopsis mutant, cad1 (constitutively activated cell death 1), which shows a phenotype that mimics the lesions seen in the hypersensitive response (HR). This mutant shows spontaneously activated expression of pathogenesis-related (PR) genes, and leading to a 32-fold increase in salicylic acid (SA). Inoculation of cad1 mutant plants with Pseudomonas syringae pv tomato DC3000 shows that the cad1 mutation results in the restriction of bacterial growth. Cloning of CAD1 reveals that this gene encodes a protein containing a domain with significant homology to the MACPF (membrane attack complex and perforin) domain of complement components and perforin proteins that are involved in innate immunity in animals. Furthermore, cell death is suppressed in transgenic cad1 plants expressing nahG, which encodes an SA-degrading enzyme. We therefore conclude that the CAD1 protein negatively controls the SA-mediated pathway of programmed cell death in plant immunity.