EivF, a factor required for transcription of the adenovirus EIV promoter, binds to an element involved in EIa-dependent activation and cAMP induction.
EivF, a factor required for transcription of the adenovirus EIV promoter, binds to an element involved in EIa-dependent activation and cAMP induction.
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EivF 是腺病毒 EIV 启动子转录所需的因子，它与参与 Ela 依赖性激活和 cAMP 诱导的元件结合。
DOI：
10.1101/gad.2.8.975
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发表时间：
1988
影响因子：
10.5
通讯作者：
Reinberg,D
中科院分区：
文献类型：
--
作者：
Cortes,P;Buckbinder,L;Leza,MA;Rak,N;Hearing,P;Merino,A;Reinberg,D
描述了从腺病毒早期基因IV的启动子（一种Ela激活的启动子）转录特异性所需的细胞因子的分离。使用功能性转录测定从HeLa细胞中纯化该因子（EivF），并鉴定为72，000 - 65，000-道尔顿蛋白。DNA酶I足迹法实验表明，纯化的EivF结合到存在于Eiv TATA盒上游两次的序列5 '-GT（G/T）ACGT-3'。从HeLa细胞制备的核提取物含有一个以上的因子能够结合到EivF识别位点。先前的研究表明，与EivF结合位点相似的序列被43，000-道尔顿蛋白识别，并参与生长抑素启动子的cAMP反应。纯化和转录活性EivF也结合到DNA序列元件中存在的生长抑素和α-促性腺激素启动子先前显示响应cAMP。
The isolation of a cellular factor that was specifically required for transcription from the promoter of the adenovirus early gene iv, an EIa-activated promoter, is described. This factor (EivF) was purified from HeLa cells using a functional transcription assay and identified as a 72,000- to 65,000-dalton protein. DNase I footprinting experiments demonstrated that purified EivF bound to the sequence 5'-GT(G/T)ACGT-3' present two times upstream of the Eiv TATA box. Nuclear extracts prepared from HeLa cells contained more than one factor capable of binding to the EivF recognition site. Previous studies have indicated that a sequence similar to the EivF-binding site was recognized by a 43,000-dalton protein and participated in the cAMP response of the somatostatin promoter. The purified and transcriptionally active EivF also bound to DNA sequence elements present in the somatostatin and alpha-gonadotropin promoters shown previously to be responsive to cAMP.