Phylogenetic diversity of the Bacillus pumilus group and the marine ecotype revealed by multilocus sequence analysis.
Phylogenetic diversity of the Bacillus pumilus group and the marine ecotype revealed by multilocus sequence analysis.
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多焦点序列分析揭示了pumilus杆菌和海洋生态型的系统发育多样性。
DOI：
10.1371/journal.pone.0080097
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Shao Z
中科院分区：
文献类型：
--
作者：
Liu Y;Lai Q;Dong C;Sun F;Wang L;Li G;Shao Z
与短小芽孢杆菌密切相关的细菌不能与诸如B等其他物种区分开来。safensis，B. stratosphericus，B. B. altitudinis和B. 16 S rRNA基因序列测定。本研究以7个管家基因为基础，对76株海洋菌株进行系统发育分析，以了解其与其他来源菌株的亲缘关系。构建了基于7个管家基因的系统发育树，顺序为gyrB-rpoB-pycA-pyrE-mutL-aroE-trpB，并与基于单个基因的树进行了比较。所有这些树表现出相似的拓扑结构，变化不大。79株菌从A到F分为6个群，其中A群最大，包含49株菌，与B群接近。altitudinis。B提供了另外两个大组。safensis和B. pumilus分别。在管家基因中，gyrB和pyrE表现出相对较好的分辨率，可以作为分子标记来区分这些密切相关的菌株。此外，基于gyrB基因的重组系统发育树的基础上，包括73个陆地和我们的分离物，海洋和其他来源的关系进行了检测。这棵树清楚地表明，海洋来源的细菌在所有大的群体中聚集在一起。相反，属于B. safensis主要由陆生细菌组成。有趣的是，几乎所有的海洋分离株都位于树的顶部，这表明该细菌群在海洋环境中最近出现分歧的可能性。我们得出结论，B. altitudinis细菌是传播最广泛的B。pumilus组在海洋环境中。总之，这份报告提供了关于这一细菌群体系统进化的第一个证据，了解它们的系统发育多样性将有助于了解它们在海洋环境中的生态作用和分布。
Bacteria closely related to Bacillus pumilus cannot be distinguished from such other species as B. safensis, B. stratosphericus, B. altitudinis and B. aerophilus simply by 16S rRNA gene sequence. In this report, 76 marine strains were subjected to phylogenetic analysis based on 7 housekeeping genes to understand the phylogeny and biogeography in comparison with other origins. A phylogenetic tree based on the 7 housekeeping genes concatenated in the order of gyrB-rpoB-pycA-pyrE-mutL-aroE-trpB was constructed and compared with trees based on the single genes. All these trees exhibited a similar topology structure with small variations. Our 79 strains were divided into 6 groups from A to F; Group A was the largest and contained 49 strains close to B. altitudinis. Additional two large groups were presented by B. safensis and B. pumilus respectively. Among the housekeeping genes, gyrB and pyrE showed comparatively better resolution power and may serve as molecular markers to distinguish these closely related strains. Furthermore, a recombinant phylogenetic tree based on the gyrB gene and containing 73 terrestrial and our isolates was constructed to detect the relationship between marine and other sources. The tree clearly showed that the bacteria of marine origin were clustered together in all the large groups. In contrast, the cluster belonging to B. safensis was mainly composed of bacteria of terrestrial origin. Interestingly, nearly all the marine isolates were at the top of the tree, indicating the possibility of the recent divergence of this bacterial group in marine environments. We conclude that B. altitudinis bacteria are the most widely spread of the B. pumilus group in marine environments. In summary, this report provides the first evidence regarding the systematic evolution of this bacterial group, and knowledge of their phylogenetic diversity will help in the understanding of their ecological role and distribution in marine environments.