SEQUENTIAL TRANSCRIPTION OF GENES OF VESICULAR STOMATITIS-VIRUS
SEQUENTIAL TRANSCRIPTION OF GENES OF VESICULAR STOMATITIS-VIRUS
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DOI：
10.1073/pnas.73.5.1504
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
BANERJEE, AK
中科院分区：
文献类型：
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作者：
ABRAHAM, G;BANERJEE, AK
水泡性口炎病毒颗粒暴露于UV辐射的增加导致编码病毒结构蛋白L、G、M、NS和N的单个mRNA种类的体外合成的差异抑制。每种mRNA的合成均表现为单次命中动力学。每个基因的表达的目标大小来自动力学数据，并发现编码N蛋白的mRNA的分子量与其相应基因的目标大小成比例。剩下的基因的目标大小比预期的要大，范围高达水泡性口炎病毒整个基因组的分子量。显然，RNA转录起始于RNA基因组上的单个位点，并且单个mRNA种类是顺序合成的。该分析允许以5“-L-G-（M-NS）-N-3”的顺序定位该病毒的基因。
Increasing exposure of vesicular stomatitis virus particles to UV radiation caused differential inhibition of the synthesis in vitro of individual mRNA species which code for the viral structural proteins L, G, M, NS and N. The synthesis of each mRNA species showed single hit kinetics. Target sizes for the expression of each gene were derived from kinetic data, and the molecular weight of the mRNA coding for the N protein was found to be proportional to the target size of its corresponding gene. The remaining genes had target sizes larger than expected, ranging up to the molecular weight of the entire genome of the vesicular stomatitis virus. Apparently RNA transcription is initiated at a single site on the RNA genome and individual mRNA species are synthesized sequentially. This analysis allowed the mapping of the genes of this virus in the order 5''-L-G-(M-NS)-N-3''.