Phenotypic, genetic, and molecular function of msc-2, a genic male sterile mutant in pepper (Capsicum annuum L.)
Phenotypic, genetic, and molecular function of msc-2, a genic male sterile mutant in pepper (Capsicum annuum L.)
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辣椒基因雄性不育突变体 MSC-2 的表型、遗传和分子功能（Capsicum annuum L.）
DOI：
10.1007/s00122-019-03510-1
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发表时间：
2019-12-20
影响因子：
5.4
通讯作者：
Shen, Huolin
中科院分区：
文献类型：
--
作者：
Cheng, Qing;Li, Ting;Shen, Huolin
大量分离分析和精细定位将辣椒基因雄性不育（msc-2）位点划分在5号染色体上一个336kb的区域。在该区域发现了一个与AtMS1同源的强候选基因Capana05g000766。基因雄性不育（msc-2）在中国北方被用来生产杂交种子。然而，目前还没有共分离标记的报道，也没有克隆出控制该性状的候选基因。本研究利用F-2群体和18Q5431AB系的大量分离分析和基因分型对msc-2进行了精细定位，并将其划分为336kb的区域。在该区域，Capana05g000766与编码参与绒毡层发育的植物同源结构域指的AtMS1同源。Capana05g000766位点的“T”缺失导致过早终止密码子，这可能导致功能丧失突变。实时荧光定量PCR分析显示，Capana05g000766是一个花药特异性基因，该基因的下调导致雄性不育。因此，Capana05g000766被确定为msc-2位点的最强候选基因。等位基因分析表明，msc-1和msc-2为非等位基因，双分子荧光互补分析表明，这两个基因在蛋白水平上不直接相互作用。由于msc-1和msc-2是拟南芥中AtDYT1和AtMS1的同源基因，它们可能在基因雄性不育辣椒的绒毡层发育中起着相似的作用，并且msc-1可能在调控绒毡层发育的其他基因中处于msc-2的上游。
Key message Bulked segregant analysis and fine mapping delimited the pepper genic male sterile (msc-2) locus into a 336 kb region on chromosome 5. A strong candidate gene, Capana05g000766, a homolog of AtMS1, was indentified in this region. Genic male sterility (msc-2) is used to produce hybrid seeds in Northern China. However, no co-segregated markers have been reported or candidate genes controlling this trait have been cloned. Here, bulked segregant analysis and genotyping of an F-2 population and a 18Q5431AB line were employed to fine map msc-2, which was delimited to a 336 kb region. In this region, Capana05g000766 was a homolog of AtMS1, which encodes a plant homeodomain finger involved in tapetum development. A "T" deletion in the Capana05g000766 locus leads to a premature stop codon, which may cause a loss-of-function mutation. Real-time PCR analysis revealed that Capana05g000766 was an anther-specific gene and down-regulation of the gene resulted in male sterility. Therefore, Capana05g000766 was identified as the strongest candidate gene for the msc-2 locus. Allelism tests showed that msc-1 and msc-2 were nonallelic, and bimolecular fluorescence complementation analysis indicated that the two genes did not interact directly with each other at the protein level. As msc-1 and msc-2 are homologs of AtDYT1 and AtMS1 in Arabidopsis, they may play similar roles in tapetum development in genic male sterile peppers, and Msc-1 might be up stream of Msc-2 in the regulation of other genes involved in tapetum development.