Expression profiling of circulating miRNAs in mouse serum in response to Echinococcus multilocularis infection
Expression profiling of circulating miRNAs in mouse serum in response to Echinococcus multilocularis infection
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小鼠血清中响应多房棘球绦虫感染的循环 miRNA 的表达谱
DOI：
10.1017/s0031182017000300
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发表时间：
2017-07-01
期刊：
影响因子：
2.4
通讯作者：
Zheng, Yadong
中科院分区：
文献类型：
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作者：
Guo, Xiaola;Zheng, Yadong
摘要多房棘球绦虫是一种致病性最强的人畜共患绦虫，可引起人类和动物的毁灭性棘球蚴病。循环microRNA（microRNAs，miRNAs）在蠕虫感染过程中稳定存在于哺乳动物宿主的血清/血浆中。在这项研究中，我们比较了宿主循环miRNA在E.多室病毒感染和未感染的小鼠。共发现58个宿主血清miRNAs差异表达（2.5倍变化，P <0.05），其中21个上调，37个显著下调。定量聚合酶链反应（PCR）结果显示，在大肠杆菌中，4种miRNAs的表达水平逐渐升高，1种表达水平逐渐降低，与测序结果一致。多房感染时间点。此外，E.在血清中鉴定了多房性贫血特异性miRNA。Real-time PCR分析进一步表明，在所有感染E.多房的这些发现将有助于理解miRNAs在宿主-寄生虫相互作用中的作用，并增强血清miRNAs作为棘球蚴病诊断靶点的可能性。
SUMMARY Echinococcus multilocularis is a most pathogenic zoonotic tapeworm that causes devastating echinococcosis in both humans and animals. Circulating microRNAs (miRNAs) are stably existed in the serum/plasma of mammalian hosts during helminthic infection. In this study, we compared the host-circulating miRNA expression in the sera from the E. multilocularis-infected and uninfected mice. A total of 58 host-origin serum miRNAs were differentially expressed (2 ⩾ fold change, P < 0·05), of which 21 were upregulated and 37 were significantly downregulated. Consistent with the sequencing data, quantitative polymerase chain reaction (PCR) results showed that the expression levels of four miRNAs were elevated gradually and one decreased gradually at the E. multilocularis infection time points. Moreover, seven of E. multilocularis specific miRNAs were identified in the sera. Real-time PCR analyses further demonstrated that only two parasite-derived miRNAs (emu-miR-10 and emu-miR-227) were specifically amplified in all the sera from mice infected with E. multilocularis. These findings will be helpful to understand the roles of miRNAs in host–parasite interaction and to potentiate serum miRNAs as diagnostic targets for echinococcosis.