Solid and liquid media for isolating and cultivating acidophilic and acid-tolerant sulfate-reducing bacteria
Solid and liquid media for isolating and cultivating acidophilic and acid-tolerant sulfate-reducing bacteria
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DOI：
10.1093/femsle/fnw083
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发表时间：
2016-05-01
影响因子：
2.1
通讯作者：
Johnson, D. Barrie
中科院分区：
文献类型：
--
作者：
Nancucheo, Ivan;Rowe, Owen F.;Johnson, D. Barrie
生长培养基的开发有助于从环境和工业样品中富集和分离嗜酸和耐酸硫酸盐还原菌 (aSRB)，并允许其在体外培养。 “标准”固体和液体设计介质的主要特征如下：（i）使用甘油而不是脂肪酸作为电子供体； (ii) 加入化学计量浓度的锌离子以缓冲 pH 值并将 aSRB 产生的潜在有害的硫化氢转化为不溶性硫化锌； (iii) 在双层（覆盖）固体培养基的凝胶底层中加入芳香酸菌（一种不代谢甘油或酵母提取物的异养嗜酸菌），以除去aSRB产生的乙酸，该乙酸不完全氧化甘油以及所用胶凝剂（琼脂糖）的酸水解释放的脂肪酸（主要是丙酮酸）。由于 aSRB 的金属硫化物沉淀物的沉积和积累，aSRB 的菌落很容易与其他厌氧菌的菌落区分开来。所提供的数据说明了所描述的覆盖固体介质从酸性厌氧沉积物和低 pH 值硫化生物反应器中分离 aSRB 的有效性。
Growth media have been developed to facilitate the enrichment and isolation of acidophilic and acid-tolerant sulfate-reducing bacteria (aSRB) from environmental and industrial samples, and to allow their cultivation in vitro. The main features of the 'standard' solid and liquid devised media are as follows: (i) use of glycerol rather than an aliphatic acid as electron donor; (ii) inclusion of stoichiometric concentrations of zinc ions to both buffer pH and to convert potentially harmful hydrogen sulphide produced by the aSRB to insoluble zinc sulphide; (iii) inclusion of Acidocella aromatica (an heterotrophic acidophile that does not metabolize glycerol or yeast extract) in the gel underlayer of double layered (overlay) solid media, to remove acetic acid produced by aSRB that incompletely oxidize glycerol and also aliphatic acids (mostly pyruvic) released by acid hydrolysis of the gelling agent used (agarose). Colonies of aSRB are readily distinguished from those of other anaerobes due to their deposition and accumulation of metal sulphide precipitates. Data presented illustrate the effectiveness of the overlay solid media described for isolating aSRB from acidic anaerobic sediments and low pH sulfidogenic bioreactors.