RNA polymerase II associated proteins regulate stomatal development through direct interaction with stomatal transcription factors in Arabidopsis thaliana
RNA polymerase II associated proteins regulate stomatal development through direct interaction with stomatal transcription factors in Arabidopsis thaliana
复制标题
RNA 聚合酶 II 相关蛋白通过与拟南芥气孔转录因子直接相互作用来调节气孔发育。
DOI：
10.1111/nph.17004
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发表时间：
2020-11-23
期刊：
影响因子：
9.4
通讯作者：
Hou，Suiwen
中科院分区：
文献类型：
--
作者：
Chen，Liang;Zhao，Mingfeng;Hou，Suiwen
RNA聚合酶II（Pol II）相关蛋白（RPAP）在细胞水平上具有多种功能，但其在酵母、动物和植物发育过程中的作用却知之甚少，通过对编码Pol II第三大亚基的NRPB 3相互作用子的筛选，我们鉴定出哺乳动物RPAP 2的直向同源物RIMA。结合遗传和生化分析揭示了RIMA和其他RPAP在拟南芥气孔发育中的作用，我们发现RIMA参与NRPB 3和其他Pol II亚基的核输入，并且在气孔细胞谱系中抑制分裂和建立细胞身份是必不可少的。此外，与RIMA相互作用的植物RPAP IYO/RPAP 1和QQT 1/RPAP 4对气孔发育也至关重要。重要的是，RIMA和QQT 1与气孔转录因子SPEECHLESS、MUTE、法马和SCREAMs物理结合。RIMA-QQT 1-IYO复合物可以与关键的气孔转录因子和Pol II一起工作，以驱动气孔细胞谱系中的细胞命运转变。与气孔转录因子的直接相互作用提供了一种新的机制，RPAP蛋白可以控制真核生物的细胞类型和组织的分化。
RNA polymerase II (Pol II) associated proteins (RPAPs) have been ascribed diverse functions at the cellular level; however, their roles in developmental processes in yeasts, animals and plants are very poorly understood.Through screening for interactors of NRPB3, which encodes the third largest subunit of Pol II, we identified RIMA, the orthologue of mammalian RPAP2. A combination of genetic and biochemical assays revealed the role of RIMA and other RPAPs in stomatal development inArabidopsis thaliana.We show that RIMA is involved in nuclear import of NRPB3 and other Pol II subunits, and is essential for restraining division and for establishing cell identity in the stomatal cell lineage. Moreover, plant RPAPs IYO/RPAP1 and QQT1/RPAP4, which interact with RIMA, are also crucial for stomatal development. Importantly, RIMA and QQT1 bind physically to stomatal transcription factors SPEECHLESS, MUTE, FAMA and SCREAMs.The RIMA‐QQT1‐IYO complex could work together with key stomatal transcription factors and Pol II to drive cell fate transitions in the stomatal cell lineage. Direct interactions with stomatal transcription factors provide a novel mechanism by which RPAP proteins may control differentiation of cell types and tissues in eukaryotes.