Molecular anatomy of a trafficking organelle
Molecular anatomy of a trafficking organelle
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DOI：
10.1016/j.cell.2006.10.030
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发表时间：
2006-11-17
期刊：
影响因子：
64.5
通讯作者：
Jahn, Reinhard
中科院分区：
文献类型：
--
作者：
Takamori, Shigeo;Holt, Matthew;Jahn, Reinhard
真核细胞中的膜运输涉及从供体隔室出芽并与受体隔室融合的囊泡的运输。出芽和融合的共同原则已经出现，许多参与这些事件的蛋白质现在已经知道。然而，目前还没有关于整个贩运细胞器的详细资料。使用突触囊泡作为模型，我们现在已经确定了蛋白质和脂质的组成;测量囊泡的大小，密度和质量;计算每个囊泡的平均蛋白质和脂质质量;并确定了十几种主要成分的拷贝数。已经构建了一个模型，整合了所有的定量数据，包括丰富的蛋白质的结构模型。突触囊泡由蛋白质主导，具有令人惊讶的运输蛋白多样性，并且除了仅以一到两个拷贝存在的V-ATP酶之外，还包含许多拷贝的膜运输和神经递质摄取所必需的蛋白质。
Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.