Analysis of Pseudomonas aeruginosa Motility in Microchannels
Analysis of Pseudomonas aeruginosa Motility in Microchannels
复制标题
铜绿假单胞菌在微通道中的运动分析
DOI：
10.9713/kcer.2012.50.4.743
复制
发表时间：
2012
影响因子：
2.7
通讯作者：
Chang
中科院分区：
文献类型：
--
作者：
Sung;Heon;Chang
本研究以铜绿假单胞菌为研究对象，研究了不同微结构对其游泳行为的影响。首先，我们测量了单细胞在二维空间下的运动参数，包括细胞速度、运行持续时间和滚动角。结果被用来计算从10到10的微通道宽度内的运动系数。由于测量的单细胞运动参数依赖于鞭毛与微通道壁的相互作用，因此细胞在受限几何形状中运行的持续时间明显不同。因此，细菌的运动性被限制的几何形状所降低。本研究表明，微流控技术是在有限的空间条件下分析细菌运动的有效工具和快速的分析工具。
This study presents the effects of micro-geometries on the swimming behavior of Pseudomonas aeruginosa. First, we have measured parameters of single-cell motility including cell speed, run duration time, and tumble angle under two dimensional space. The results are used to calculate motility coefficients in the width of microchannels ranging from 10 to . Since the single-cell motility parameters measured depend on the interaction of flagella with the microchannel wall, the duration time of the running cell in restricted geometries is distinctively different. Therefore, the motility of bacteria is decreased by restricted geometries. This study suggests that microfluidic approach is useful tool for the analysis of bacterial motility under the restricted space and rapid analytical tool.