Positive regulation of endochondral cartilage growth by perichondrial and periosteal calcitonin
Positive regulation of endochondral cartilage growth by perichondrial and periosteal calcitonin
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DOI：
10.1210/en.2002-220905
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发表时间：
2003-05-01
期刊：
影响因子：
4.8
通讯作者：
Linsenmayer, TF
中科院分区：
文献类型：
--
作者：
Di Nino, DL;Linsenmayer, TF
我们以前的研究表明，在胚胎发育过程中的鸟类长骨，软骨成分的生长是由周围的软骨膜（PC）和骨膜（PO）分泌的多种因子调节。这些因子的活动，其中包括积极和消极的调节，可以检测到条件培养基PC和PO细胞培养。在本研究中，我们已经获得的证据表明，一个积极的调节剂是肽激素降钙素（CT）。通过条件培养基的质谱分析，其中一种组分的分子量为3.4 kDa，相当于鸡CT的大小。通过RT-PCR，组织和细胞培养物含有CT的mRNA，并且通过免疫组织化学，细胞含有蛋白质。进一步的免疫组织化学分析表明，这种蛋白质是正常分泌的，这表明用莫能菌素（一种阻断胞吐作用的化合物）处理的细胞含有升高的细胞内CT。在功能上，CT的长骨雏形的器官培养物的效果增加的增长，以类似的PC和PO条件培养基的方式。总之，这些数据表明，PC和PO分泌CT以旁分泌方式对下层软骨的生长产生积极刺激作用。
Our previous studies showed that during the embryonic development of avian long bones, growth of the cartilaginous component is regulated by multiple factors secreted by the surrounding perichondrium (PC) and periosteum (PO). The activities of these factors-which include both positive and negative regulators-can be detected in conditioned media from PC and PO cell cultures. In the present study, we have obtained evidence suggesting that a positive regulator is the peptide hormone calcitonin (CT). By mass spectrometry of conditioned media, one of the components has a molecular mass of 3.4 kDa, the size of chicken CT. By RT-PCR the tissue and cell cultures contain mRNA for CT, and by immunohistochemistry the cells contain the protein. That the protein is normally secreted is suggested by further immunohistochemical analyses, which show that cells treated with monensin, a compound that blocks exocytosis, contain elevated intracellular CT. Functionally, the addition of CT to organ cultures of long bone rudiments effects increased growth in a manner similar to that of the PC- and PO-conditioned media. Taken together, these data suggest that secretion of CT by the PC and PO effects, in a paracrine manner, positive stimulation of growth in the underlying cartilage.