Fermentation, Hydrogen, and Sulfur Metabolism in Multiple Uncultivated Bacterial Phyla
Fermentation, Hydrogen, and Sulfur Metabolism in Multiple Uncultivated Bacterial Phyla
复制标题
DOI：
10.1126/science.1224041
复制
发表时间：
2012-09-28
期刊：
影响因子：
56.9
通讯作者：
Banfield, Jillian F.
中科院分区：
文献类型：
--
作者：
Wrighton, Kelly C.;Thomas, Brian C.;Banfield, Jillian F.
BD1-5、OP11 和 OD1 细菌已在厌氧环境中广泛检测到，但由于缺乏培养代表和基因组采样最少，它们的代谢仍不清楚。通过从醋酸盐修正的含水层中独立培养地恢复 49 个部分至接近完整的基因组，我们发现了这些门成员的代谢特征，以及一个新的谱系 PER。所有生物体都是预计会发酵的非呼吸厌氧菌。其中三种利用类似古菌的杂种 II/III 型核酮糖-1,5-二磷酸羧化酶-加氧酶 (RuBisCO) 增强发酵，该酶将单磷酸腺苷回收与 CO2 固定结合起来，这是细菌中以前未描述过的途径。 OD1 的成员可以还原硫并可以使用古细菌型氢化酶泵送质子。对于六种生物体，UGA 终止密码子被翻译为色氨酸。这里研究的所有细菌可能在氢气生产、硫循环和难熔沉积碳发酵中发挥着以前未被认识到的作用。
BD1-5, OP11, and OD1 bacteria have been widely detected in anaerobic environments, but their metabolisms remain unclear owing to lack of cultivated representatives and minimal genomic sampling. We uncovered metabolic characteristics for members of these phyla, and a new lineage, PER, via cultivation-independent recovery of 49 partial to near-complete genomes from an acetate-amended aquifer. All organisms were nonrespiring anaerobes predicted to ferment. Three augment fermentation with archaeal-like hybrid type II/III ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) that couples adenosine monophosphate salvage with CO2 fixation, a pathway not previously described in Bacteria. Members of OD1 reduce sulfur and may pump protons using archaeal-type hydrogenases. For six organisms, the UGA stop codon is translated as tryptophan. All bacteria studied here may play previously unrecognized roles in hydrogen production, sulfur cycling, and fermentation of refractory sedimentary carbon.