The role of African buffalos (syncerus caffer) in the maintenance of foot-and-mouth disease in Uganda
The role of African buffalos (syncerus caffer) in the maintenance of foot-and-mouth disease in Uganda
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DOI：
10.1186/1746-6148-6-54
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发表时间：
2010-12-11
影响因子：
2.6
通讯作者：
Alexandersen, Soren
中科院分区：
文献类型：
--
作者：
Ayebazibwe, Chrisostom;Mwiine, Frank N.;Alexandersen, Soren
背景资料：研究非洲水牛的作用（Syncerus caffer）在乌干达口蹄疫的维持中，从207头非洲水牛、21头黑斑羚和21头非洲水牛中收集血清样品，（Aepyceros melampus），1只长颈鹿（长颈鹿camelopardalis），1只普通大羚羊（Taurotragus oryx），2005年至2008年期间，乌干达四个主要国家公园的7只角马（Alcelaphus buselaphus）和5只水羚（Kobus ellipsiprymnus）。使用Ceditest（R）FMDV NS ELISA筛选血清样品以检测针对口蹄疫病毒（FMDV）非结构蛋白（NSP）的抗体。用固相阻断ELISA法（SPBE）检测阳性标本中7种血清型抗体的型特异性。结果：在所检测的布法罗样品中，85%的水牛样品中，有100%的水牛样品中有1000%的水牛样品中有100（95% CI = 80-90%）的FMDV非结构蛋白抗体阳性，而7个样本中有1个样本的FMDV非结构蛋白抗体阳性。（14.3%; 95% CI = -11.6-40.2%）是来自各种不同物种的35份其他野生动物样本中唯一的阳性。在布法罗中，针对血清型O（7/27个样品）、SAT 1（23/29个样品）、SAT 2（18/32个样品）和SAT 3（16/30个样品）发现了高的血清型特异性抗体滴度（≥ 80）。在针对四种血清型O、SAT 1、SAT 2和SAT 3的抗体滴定的样品中，17/22（77%; CI = 59.4-94.6%）具有针对至少两种血清型的高滴度。从2007年在伊丽莎白女王国家公园（QENP）收集的布法罗先证样本中获得血清型SAT 1（1个样本）和SAT 2（2个样本）的FMDV分离株。序列分析和VP 1编码序列的比较表明，SAT 1株属于拓扑型IV，而SAT 2株属于不同的血统在东非拓扑型X的结论：一致检测水牛高抗体滴度支持的观点，非洲水牛发挥重要作用，在乌干达国家公园内的口蹄疫病毒感染的维护。SAT 1和SAT 2病毒均被分离，血清学数据表明，布法罗群体中也可能存在FMDV血清型O和SAT 3。应开展详细研究，进一步确定野生动物在东非口蹄疫流行病学中的作用。
Background: To study the role of African buffalos (Syncerus caffer) in the maintenance of foot-and-mouth disease in Uganda, serum samples were collected from 207 African buffalos, 21 impalas (Aepyceros melampus), 1 giraffe (Giraffa camelopardalis), 1 common eland (Taurotragus oryx), 7 hartebeests (Alcelaphus buselaphus) and 5 waterbucks (Kobus ellipsiprymnus) from four major National Parks in Uganda between 2005 and 2008. Serum samples were screened to detect antibodies against foot-and-mouth disease virus (FMDV) non-structural proteins (NSP) using the Ceditest (R) FMDV NS ELISA. Solid Phase Blocking ELISAs (SPBE) were used to determine the serotype-specificity of antibodies against the seven serotypes of FMDV among the positive samples. Virus isolation and sequencing were undertaken to identify circulating viruses and determine relatedness between them.Results: Among the buffalo samples tested, 85% (95% CI = 80-90%) were positive for antibodies against FMDV non-structural proteins while one hartebeest sample out of seven (14.3%; 95% CI = -11.6-40.2%) was the only positive from 35 other wildlife samples from a variety of different species. In the buffalo, high serotype-specific antibody titres (= 80) were found against serotypes O (7/27 samples), SAT 1 (23/29 samples), SAT 2 (18/32 samples) and SAT 3 (16/30 samples). Among the samples titrated for antibodies against the four serotypes O, SAT 1, SAT 2 and SAT 3, 17/22 (77%; CI = 59.4-94.6%) had high titres against at least two serotypes. FMDV isolates of serotypes SAT 1 (1 sample) and SAT 2 (2 samples) were obtained from buffalo probang samples collected in Queen Elizabeth National Park (QENP) in 2007. Sequence analysis and comparison of VP1 coding sequences showed that the SAT 1 isolate belonged to topotype IV while the SAT 2 isolates belonged to different lineages within the East African topotype X.Conclusions: Consistent detection of high antibody titres in buffalos supports the view that African buffalos play an important role in the maintenance of FMDV infection within National Parks in Uganda. Both SAT 1 and SAT 2 viruses were isolated, and serological data indicate that it is also likely that FMDV serotypes O and SAT 3 may be present in the buffalo population. Detailed studies should be undertaken to define further the role of wildlife in the epidemiology of FMDV in East Africa.