Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis
Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis
复制标题
DOI：
10.1073/pnas.95.17.10306
复制
发表时间：
1998-08-18
影响因子：
11.1
通讯作者：
Parker, JE
中科院分区：
文献类型：
--
作者：
Aarts, N;Metz, M;Parker, JE
拟南芥基因EDS 1和NDR 1先前通过突变分析显示编码种族特异性抗病性的重要组成部分。在这里，我们研究了EDS 1和NDR 1的相对需求，广泛的抗性（R）基因存在于三个拟南芥品种（哥伦比亚，Landsberg-erecta，和Wassilewskija）。我们发现，有一个强烈的需求EDS 1的一个子集的R基因座（RPP 2，RPP 4，RPP 5，RPP 21，和RPS 4），赋予抗生物营养卵菌霜霉寄生，假单胞菌表达无毒基因avrRps 4。这些EDS 1依赖性R基因座对NDR 1的需求很弱或不可测量。相反，三个NDR 1依赖性R基因座，RPS 2，RPM 1和RPS 5，独立于EDS 1运作。另一个RPP基因座RPP 8在对寄生疫霉的分离物特异性抗性中对EDS 1或NDR 1没有强烈的排他性需求，尽管抗性被EDS 1微弱地损害。类似地，由两个EDS 1依赖的RPP基因，RPP 4和RPP 5，抗性条件的NDR 1部分受损，暗示一定程度的通路串扰。我们的研究结果提供了令人信服的证据，为特定的R基因优先利用任一信号组分，从而定义至少两个抗病途径。这些数据还表明，对EDS 1或NDR 1的强烈依赖是由R蛋白结构类型而不是病原体类别决定的。
The Arabidopsis genes EDS1 and NDR1 were shown previously by mutational analysis to encode essential components of race-specific disease resistance. Here, we examined the relative requirements for EDS1 and NDR1 by a broad spectrum of Resistance (R) genes present in three Arabidopsis accessions (Columbia, Landsberg-erecta, and Wassilewskija). We show that there is a strong requirement for EDS1 by a subset of R loci (RPP2, RPP4, RPP5, RPP21, and RPS4), conferring resistance to the biotrophic oomycete Peronospora parasitica, and to Pseudomonas bacteria expressing the avirulence gene avrRps4. The requirement for NDR1 by these EDS1-dependent R loci is either weak or not measurable. Conversely, three NDR1-dependent R loci, RPS2, RPM1, and RPS5, operate independently of EDS1. Another RPP locus, RPP8, exhibits no strong exclusive requirement for EDS1 or NDR1 in isolate-specific resistance to P. parasitica, although resistance is compromised weakly by eds1. Similarly, resistance conditioned by two EDS1-dependent RPP genes, RPP4 and RPP5, is impaired partially by ndr1, implicating a degree of pathway cross-talk Our results provide compelling evidence for the preferential utilization of either signaling component by particular R genes and thus define at least two disease resistance pathways. The data also suggest that strong dependence on EDS1 or NDR1 is governed by R protein structural type rather than pathogen class.