QUANTITATIVE MORPHOLOGY OF HUMAN GLIOBLASTOMA-MULTIFORME MICROVESSELS - STRUCTURAL BASIS OF BLOOD-BRAIN-BARRIER DEFECT
QUANTITATIVE MORPHOLOGY OF HUMAN GLIOBLASTOMA-MULTIFORME MICROVESSELS - STRUCTURAL BASIS OF BLOOD-BRAIN-BARRIER DEFECT
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DOI：
10.1007/bf00148386
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发表时间：
1987-01-01
影响因子：
3.9
通讯作者：
DELMAESTRO, RF
中科院分区：
文献类型：
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作者：
COOMBER, BL;STEWART, PA;DELMAESTRO, RF
脑实质的肿瘤侵袭导致血管壁的超微结构破坏，使得血清蛋白外渗到周围组织中，导致脑水肿。所涉及的结构变化尚未得到很好的理解，因为在正常屏障血管和屏障受损的肿瘤血管中，血清成分通过的孔（渗透性途径）尚未得到广泛研究。在这项研究中，我们研究了人脑微血管的超微结构在对照脑组织和五个多形性胶质母细胞瘤肿瘤活检标本。共78个血管的电子显微镜照片进行了分析与计算机辅助形态学的渗透性路线的超微结构证据。未观察到内皮中的开窗。胞饮小泡的数量和排列与对照组脑血管无显著差异。在大多数肿瘤的一些血管中观察到扩张扩大的上皮间连接（可能代表通过跨内皮通道的切片），但在对照屏障血管中未观察到。此外，在不到2%的肿瘤血管中观察到内皮层的大间隙。总之，本研究中的多形性胶质母细胞瘤血管显示出与对照组相比血管形态的细微变化。我们认为，多形性胶质母细胞瘤肿瘤中的高血管通透性和由此产生的脑水肿可能是由于存在通过内皮细胞间连接的通道，以及内皮细胞壁中罕见但较大的破裂。
Neoplastic invasion of the brain parenchyma results in a disruption of the ultrastructure of the blood vessel walls such that serum proteins extravasate into the surrounding tissue, resulting in cerebral edema. The structural changes involved are not well understood, since the pores through which serum constituents pass (permeability routes) in normal barrier vessels and in tumor vessels where the barrier is compromised, have not been extensively explored. In this study we investigate the ultrastructure of human brain microvessels in biopsied samples of control brain tissue and five glioblastoma multiforme tumors. Electron micrographs of a total of 78 vessels were analysed with computer assisted morphometry for ultrastructural evidence of permeability routes. Fenestrations in the endothelium were not seen. Pinocytotic vesicle number and arrangement did not differ significantly from that seen in control brain vessels. Interendothelial junctions with enlarged distensions (which may represent sections through transendothelial channels) were seen in some vessels from most tumors but not in control barrier vessels. In addition, large gaps in the endothelial layer were seen in less than two percent of tumor vessels. In conclusion, glioblastoma multiforme vessels in this study show subtle alterations in vessel morphology from that seen in controls. We suggest that the high vascular permeability and resultant brain edema seen in glioblastoma multiforme tumors is likely due to the presence of channels through interendothelial junctions, and rare but large breaks in the endothelial wall.