External mass transfer in high performance liquid chromatography systems.
External mass transfer in high performance liquid chromatography systems.
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DOI：
10.1016/j.chroma.2008.09.035
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发表时间：
2008-11
期刊：
影响因子：
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通讯作者：
K. Miyabe;Motoki Ando;N. Ando;G. Guiochon
中科院分区：
文献类型：
--
作者：
K. Miyabe;Motoki Ando;N. Ando;G. Guiochon
用脉冲响应实验研究了反相高效液相色谱系统中的外部传质。利用矩量法对不同流速和温度下的洗脱峰的一阶矩和二阶矩进行分析，确定了外传质系数kf。舍伍德数对雷诺数和施密特数的依赖关系与以往研究中所观察到的传统文献关联式几乎相同。最后两个无量纲参数的指数被推导为在0.28至0.41的范围内。当使用Kataoka方程时，计算出本研究中估计的kf值的均方差为0.21。它的结论是，传统的相关性可以用来估计kf值，即使当颗粒直径是微米的顺序。
External mass transfer in a HPLC system operated in the reversed-phase mode was studied by pulse response experiments, using a column packed with non-porous C18-silica gel spherical particles, 18μm in diameter. The first and second moments of the elution peaks, measured under different flow velocities and temperatures, were analyzed by the moment method to determine the external mass transfer coefficient (kf). The dependence of the Sherwood number on the Reynolds and the Schmidt numbers is almost the same as that observed in previous investigations of conventional literature correlations. The exponent of the last two nondimensional parameters was derived as being in the range from 0.28 to 0.41. When the Kataoka equation is used, the mean square deviation was calculated to be 0.21 for the values of kfestimated in this study. It is concluded that conventional correlations can be used to estimate kfvalues, even when the particle diameter is of the order of a micrometer.