Acetylation dynamics and stoichiometry in Saccharomyces cerevisiae.
Acetylation dynamics and stoichiometry in Saccharomyces cerevisiae.
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DOI：
10.1002/msb.134766
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发表时间：
2014
影响因子：
9.9
通讯作者：
Choudhary, Chunaram
中科院分区：
文献类型：
--
作者：
Weinert, Brian T.;Iesmantavicius, Vytautas;Moustafa, Tarek;Scholz, Christian;Wagner, Sebastian A.;Magnes, Christoph;Zechner, Rudolf;Choudhary, Chunaram
赖氨酸乙酰化是一种经常发生的翻译后修饰，然而，对大多数位点的起源和调控知之甚少。在这里，我们使用定量质谱分析乙酰化动力学和化学计量在酿酒酵母。我们发现，乙酰化在生长停滞细胞中以依赖于不同亚细胞区室中乙酰辅酶A产生的方式积累。线粒体乙酰化水平与体内乙酰辅酶A浓度和体外乙酰辅酶A非酶乙酰化赖氨酸残基相关。我们开发了一种方法来估计乙酰化化学计量，发现绝大多数线粒体和细胞质乙酰化具有非常低的化学计量。然而，线粒体乙酰化发生在比细胞质乙酰化显著更高的基础水平，与线粒体中不同的乙酰化动力学和更高的乙酰辅酶A浓度一致。高化学计量乙酰化主要发生在组蛋白、存在于组蛋白乙酰转移酶和去乙酰化酶复合物中的蛋白质以及转录因子上。这些数据表明，在指数生长的酵母中，大部分乙酰化发生在非常低的水平上，并且受到乙酰辅酶A暴露的一致影响。
Lysine acetylation is a frequently occurring posttranslational modification; however, little is known about the origin and regulation of most sites. Here we used quantitative mass spectrometry to analyze acetylation dynamics and stoichiometry in Saccharomyces cerevisiae. We found that acetylation accumulated in growth‐arrested cells in a manner that depended on acetyl‐CoA generation in distinct subcellular compartments. Mitochondrial acetylation levels correlated with acetyl‐CoA concentration in vivo and acetyl‐CoA acetylated lysine residues nonenzymatically in vitro. We developed a method to estimate acetylation stoichiometry and found that the vast majority of mitochondrial and cytoplasmic acetylation had a very low stoichiometry. However, mitochondrial acetylation occurred at a significantly higher basal level than cytoplasmic acetylation, consistent with the distinct acetylation dynamics and higher acetyl‐CoA concentration in mitochondria. High stoichiometry acetylation occurred mostly on histones, proteins present in histone acetyltransferase and deacetylase complexes, and on transcription factors. These data show that a majority of acetylation occurs at very low levels in exponentially growing yeast and is uniformly affected by exposure to acetyl‐CoA.
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影响因子：
56.9
作者：
Choudhary, Chunaram;Kumar, Chanchal;Mann, Matthias
通讯作者：
Mann, Matthias
DOI：
10.1074/mcp.m111.011015
发表时间：
2011-09
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Michalski A;Damoc E;Hauschild JP;Lange O;Wieghaus A;Makarov A;Nagaraj N;Cox J;Mann M;Horning S
通讯作者：
Horning S
影响因子：
4.4
作者：
Cox, Juergen;Neuhauser, Nadin;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
4.8
作者：
Albaugh, Brittany N.;Arnold, Kevin M.;Denu, John M.
通讯作者：
Denu, John M.
影响因子：
10.5
作者：
Millar, CB;Xu, F;Grunstein, M
通讯作者：
Grunstein, M