Characterization of a cell line, NKL, derived from an aggressive human natural killer cell leukemia.
Characterization of a cell line, NKL, derived from an aggressive human natural killer cell leukemia.
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发表时间：
1996-02
影响因子：
2.6
通讯作者：
M. Robertson;Keith J. Cochran;C. Cameron;J. Le;R. Tantravahi;J. Ritz
中科院分区：
文献类型：
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作者：
M. Robertson;Keith J. Cochran;C. Cameron;J. Le;R. Tantravahi;J. Ritz
从1例CD_3-CD_（16）+CD_（56）+大颗粒淋巴细胞（LGL）白血病患者的外周血中建立了NKL细胞系。该患者的肿瘤性LGL介导自然杀伤和抗体依赖性细胞毒性（ADCC），并表现出与正常CD 16 + CD 56 dim自然杀伤（NK）细胞相似的增殖反应。NKL细胞的形态类似于正常活化的NK细胞。核型为47，XY，add（1）（q42），+6 del（6）（q15 q23），del（17）（p11）。NKL细胞表达CD 2、CD 6、CD 11 a、CD 26、CD 27、CD 29、CD 38、CD 43、CD 58、CD 81、CD 94、CD 95、II类MHC和C1.7.1抗原，但在细胞表面上不表达可检测水平的CD 3、CD 4、CD 5、CD 8、CD 14、CD 19、CD 20、CD 28、α/β或γ/δ T细胞受体。在长期的体外培养过程中，NKL细胞上的CD 16、CD 56和CD 57抗原的密度显著下降。然而，NKL细胞可以介导ADCC以及自然杀伤。NKL细胞严格依赖于白细胞介素-2（IL-2）以持续生长，并且如果剥夺IL-2超过7天则死亡。NKL细胞响应低至1 pM的IL-2浓度而增殖，但最佳增殖反应需要约100 pM IL-2。在IL-2存在下生长的NKL细胞表达丰富的IL-2 R α，细胞表面上几乎没有或没有可检测的IL-2 β或γ链;缺乏IL-2的NKL细胞表达高水平的IL-2 R α和β。与IL-2不同，IL-4、IL-7和IL-12不能维持NKL细胞的活力。此外，IL-1、IL-4、IL-6、IL-7、IL-12、肿瘤坏死因子-α（TNF-α）、干扰素-α（IFN-α）和IFN-γ不支持NKL细胞的生长。NKL细胞系可证明对人类NK细胞生物学的研究有用。
Cell line NKL was established from the the peripheral blood of a patient with CD3-CD16+CD56+ large granular lymphocyte (LGL) leukemia. The neoplastic LGL of this patient mediated natural killing and antibody-dependent cellular cytotoxicity (ADCC) and exhibited proliferative responses similar to normal CD16+CD56dim natural killer (NK) cells. The Morphology of NKL cells resembles that of normal activated NK cells. The karyotype of NKL is 47, XY, add (1) (q42), +6 del (6) (q15 q23), del (17) (p11). NKL cells express CD2, CD6, CD11a, CD26, CD27, CD29, CD38, CD43, CD58, CD81, CD94, CD95, class II MHC, and the C1.7.1 antigen, but do not express detectable levels of CD3, CD4, CD5, CD8, CD14, CD19, CD20, CD28, alpha/beta or gamma/delta T cell receptors on the cell surface. The density of the CD16, CD56, and CD57 antigens declined markedly on NKL cells during prolonged im vitro culture. Nevertheless, NKL cells can mediate ADCC as well as natural killing. NKL cells are strictly dependent on interleukin-2 (IL-2) for sustained growth and die if deprived of IL-2 for more than 7 days. NKL cells proliferate in response to concentrations of IL-2 as low as 1 pM, but an optimal proliferative response requires approximately 100 pM IL-2. NKL cells growing in the presence of IL-2 express abundant IL-2R alpha with little or no detectable IL-2 beta or gamma chain on the cell surface; NKL cells deprived of IL-2 express high levels of both IL-2R alpha and beta. IL-4, IL-7, and IL-12, unlike IL-2, do not maintain the viability of NKL cells. Furthermore, IL-1, IL-4, IL-6, IL-7, IL-12, tumor necrosis factor-alpha (TNF-alpha), interferon-alpha (IFN-alpha) and IFN-gamma do not support the growth of NKL cells. The NKL cell line may prove useful for studies of human NK cell biology.