X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
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DOI：
10.1016/j.bpj.2010.12.409
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发表时间：
2011-02
影响因子：
6.1
通讯作者：
C. Putnam;M. Hammel;Greg L. Hura;J. Tainer
中科院分区：
文献类型：
--
作者：
C. Putnam;M. Hammel;Greg L. Hura;J. Tainer
蛋白质、DNA和RNA的形状、组装和相互作用的形状，加上它们详细的结构化学，编码了关于途径选择和生物结果的关键信息。我们正在开发SAXS结合晶体学作为定义大分子构象和连接的首选工具，适合于将蛋白质连接到通路和蛋白质组学规模1-2。晶体学为机械分析提供了无与伦比的结构细节;然而，它仅限于描述晶格内大分子的构象。原则上，SAXS可以提供关于小分子和大分子的可靠补充数据。在实践中，SAXS可能会受到优化样品和分析的问题的限制，这些问题可以减少或避免。我们对动态RNA，DNA和蛋白质复合物的研究结果表明，SAXS具有很大的潜力，可以提供溶液中的精确形状，构象和组装状态，并以基本方式告知生物功能4-5。
Protein, DNA, and RNA shape, assembly and interactions shapes, plus their detailed structural chemistry, encode key information regarding pathway choices and biological outcomes. We are developing SAXS combined with crystallography as a premiere tool for defining macromolecular conformations and connections suitable to join proteins to pathways and at the proteomic scale 1-2. Crystallography supplies unsurpassed structural detail for mechanistic analyses; however, it is restricted to describing conformations of macromolecules within crystal lattices. In principle, SAXS can provide reliable complementary data on small and large macromolecules 3. In practice, SAXS can be limited by problems in optimizing samples and analyses, which can be reduced or avoided. Our results on dynamic RNA, DNA, and protein complexes show that SAXS has great potential to provide accurate shapes, conformations, and assembly states in solution and inform biological functions in fundamental ways 4-5.