In vitro and in vivo performance of porcine islets encapsulated in interfacially photopolymerized poly(ethylene glycol) diacrylate membranes
In vitro and in vivo performance of porcine islets encapsulated in interfacially photopolymerized poly(ethylene glycol) diacrylate membranes
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DOI：
10.1177/096368979900800310
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发表时间：
1999-05-01
影响因子：
3.3
通讯作者：
Hubbell, JA
中科院分区：
文献类型：
--
作者：
Cruise, GM;Hegre, OD;Hubbell, JA
研究了不同浓度和分子量的聚乙二醇（PEG）二丙烯酸酯的界面光聚合形成水凝胶膜用于包封猪胰岛的有用性。本研究的结果显示了PEG包封的猪胰岛的体外和体内功能以及PEG膜在不一致异种移植模型中预防免疫排斥的能力。包封的胰岛在包封后的第一周内表现出85%的平均活力，略低于但显著低于未包封的对照组。使用静态葡萄糖刺激和灌流测定显示包封的猪胰岛是葡萄糖响应性的。在较低浓度的PEG二丙烯酸酯（10-13%）中包封的猪胰岛中观察到比在较高浓度（25%）的PEG二丙烯酸酯中包封的胰岛中观察到更高的胰岛素释放速率，相对于未包封的对照没有显著降低。灌流结果表明，双相胰岛素释放从封装胰岛响应葡萄糖刺激。链脲佐菌素诱导的糖尿病无胸腺小鼠在腹膜腔内植入5，000 - 8，000个包囊化的猪胰岛等同物后维持正常血糖长达110天。使用葡萄糖耐量试验也在这些动物中证实了正常。PEG二丙烯酸酯包封的猪胰岛在Sprague-Dawley大鼠（一种不协调异种移植模型）的腹腔中30天后显示出活力并含有胰岛素。从这些研究中，我们得出结论，PEG二丙烯酸酯封装的猪胰岛的界面光聚合显示出作为一种方法的异种保护生物人工内分泌胰腺使用的前景。
The usefulness of interfacial photopolymerization of poly(ethylene glycol) (PEG) diacrylate at a variety of concentrations and molecular weights to form hydrogel membranes for encapsulating porcine islets of Langerhans was investigated. The results from this study show in vitro and in vivo function of PEG-encapsulated porcine islets and the ability of PEG membranes to prevent immune rejection in a discordant xenograft model. Encapsulated islets demonstrated an average viability of 85% during the first week after encapsulation, slightly but significantly lower than unencapsulated controls. Encapsulated porcine islets were shown to be glucose responsive using static glucose stimulation and perifusion assays. Higher rates of insulin release were observed for porcine islets encapsulated in lower concentrations of PEG diacrylate (10-13%), not significantly reduced relative to unencapsulated controls, than were observed in islets encapsulated in higher concentrations (25%) of PEG diacrylate. Perifusion results showed biphasic insulin release from encapsulated islets in response to glucose stimulation. Streptozotocin-induced diabetic athymic mice maintained normoglycemia for up to 110 days after the implantation of 5,000-8,000 encapsulated porcine islet equivalents into the peritoneal cavity. Normoglycemia was also confirmed in these animals using glucose tolerance tests. PEG diacrylate-encapsulated porcine islets were shown to be viable and contain insulin after 30 days in the peritoneal cavity of Sprague-Dawley rats, a discordant xenograft model. From these studies, we conclude that PEG diacrylate encapsulation of porcine islets by interfacial photopolymerization shows promise for use as a method of xenoprotection toward a bioartifical endocrine pancreas.