Interaction of choline salts with artificial biological membranes: DSC studies elucidating cellular interactions.
Interaction of choline salts with artificial biological membranes: DSC studies elucidating cellular interactions.
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胆碱盐与人工生物膜的相互作用：DSC 研究阐明细胞相互作用。
DOI：
10.1016/j.bbamem.2013.03.017
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Elliott,GloriaD
中科院分区：
文献类型：
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作者：
Weaver,KatherineD;VanVorst,MatthewP;Vijayaraghavan,R;Macfarlane,DouglasR;Elliott,GloriaD
为了更好地了解稀释型离子液体的相对细胞毒性与其与生物膜的特异性相互作用之间的关系，研究了一系列胆碱型有机盐组成的模型脂膜体系的热致行为。将由二棕榈酰磷脂酰胆碱制备的单层囊泡暴露于10 mM的胆碱磷酸盐溶液中，用差示扫描量热法研究了凝胶向液晶态的转变。在以前的研究中观察到的胆碱盐具有较低的相对毒性，但对这些模型膜的脂质相变行为的影响很小。相反，胆碱双(2，4，4-三甲基戊基)磷酸盐和胆碱双(2-乙基己基)磷酸被观察到具有较高的相对毒性，导致脂类相变行为的明显破坏，这与盐类对磷脂酰链的渗透一致。胆碱双(2，4，4-三甲基戊基)磷酸胆碱降低了二棕榈酰磷脂酰胆碱的主转变的Tmand热，而双(2-乙基己基)磷酸胆碱则促进了交替相的平衡。
To better understand the relationship between the relative cytotoxicity of diluted ionic liquids and their specific interaction with biological membranes, the thermotropic behavior of model lipid membrane systems formulated in a series of choline based organic salts was investigated. Unilamellar vesicles prepared from dipalmitoylphosphatidylcholine were exposed to a series of choline phosphate salts at a concentration of 10mM at pH7.40, and the gel to liquid-crystalline state transition was examined using differential scanning calorimetry. The choline salts that were observed to have a low relative toxicity in previous studies induced minimal changes in the lipid phase transition behavior of these model membranes. In contrast, the salts choline bis(2,4,4-trimethylpentyl)phosphinate and choline bis(2-ethylhexyl)phosphate, both of which were observed to have high relative toxicity, caused distinct disruptions in the lipid phase transition behavior, consistent with penetration of the salts into the acyl chains of the phospholipids. choline bis(2,4,4-trimethylpentyl)phosphinate reduced the Tmand enthalpy of the main transition of dipalmitoylphosphatidylcholine while choline bis(2-ethylhexyl)phosphate induced the equilibration of alternate phases.