Acyl group transfer from the sn-1 position of phospholipids in the biosynthesis of n-dodecyl palmitate.
Acyl group transfer from the sn-1 position of phospholipids in the biosynthesis of n-dodecyl palmitate.
复制标题
棕榈酸正十二烷基酯生物合成中酰基从磷脂的 sn-1 位转移。
DOI：
10.1021/bi00071a018
复制
发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Rando,RR
中科院分区：
文献类型：
--
作者：
Furuyoshi,S;Shi,YQ;Rando,RR
方法L1-[9，10- 3 H，3 H] Pa！丝裂酰-2-戊基-sn-甘油基-3-磷酸胆碱。向小瓶中加入200 μ L [9，10- 3 H，3 H]棕榈酸（60.0 Ci/mmol，0.083 µ/mL）和20 µ棕榈酸（7.41 µ）。在氮气下蒸发溶剂;加入100 μ L无水苯并再次蒸发溶剂。将该循环重复三次。加入100微升草酰氯，并将溶液在氮气下于37 ℃温育4小时.然后通过氮气除去过量的草酰氯;加入100 μ L无水苯，然后蒸发溶剂。将总共200 μ L的溶血素衍生物1 -2-戊基-IM-甘油基-3-磷酸胆碱（10 mM）（Hirth et al. '，1983; Chaudrakumar & Hajdu，1983）加入到无水氯仿中，沿着加入催化量的对-（二甲基-氨基）吡啶。将混合物在37 ℃下加热12小时.产物L-1-[9，10- 3 H，3 H]棕榈酰基-2-戊基-sn-甘油基-
MethodsSynthesis of Ll-[9, 10-3H, 3H] Pa! mitoyl-2-pentyl-sn-glyceryl-3-phosphocholine. To a vial was added 200 pL of [9, 10-3H, 3H] palmitic acid (60.0 Ci/mmol, 0.083 µ/mL) and 20 µ of palmitic acid (7.41 µ). The solvent was evaporated under nitrogen; 100 pL of dry benzene was added and the solvent was evaporated again. This cycle was repeated three times. One hundred microliters of oxalyl chloride was added and the solution was incubated for 4 hat 37 C under nitrogen. The excess oxalyl chloride was then removed by nitrogen; 100 pL of dry benzene was added, followed by the evaporation of solvent. A total of 200 µ of the lysolecithin derivative l-2-pentyl-íM-glyceryl-3-phosphocholine (10 mM)(Hirth et al.', 1983; Chaudrakumar & Hajdu, 1983) was added indry chloroform, along with a catalytic amount of p-(dimethyl-amino) pyridine. The mixture was heated for 12 h at 37 C. The product L-1-[9, 10-3H, 3H] palmitoyl-2-pentyl-s «-glyceryl-