THE STRINGENT RESPONSE IN STREPTOMYCES-COELICOLOR A3(2)
THE STRINGENT RESPONSE IN STREPTOMYCES-COELICOLOR A3(2)
复制标题
DOI：
10.1111/j.1365-2958.1991.tb02109.x
复制
发表时间：
1991-02-01
影响因子：
3.6
通讯作者：
BIBB, MJ
中科院分区：
文献类型：
--
作者：
STRAUCH, E;TAKANO, E;BIBB, MJ
在抗生素生产链霉菌coelicolor A3(2)中，通过氨基酸消耗（营养下降）或添加丝氨酸羟酸盐引起了严格的反应；两者都导致ppGpp水平升高，rnd rRNA基因组的四个启动子转录减少。未经处理的批量培养分析显示，在指数增长结束时，在抗生素生产开始之前，ppGpp水平升高。通过对放线鸟素生物合成簇的早期基因actIII的S1核酸酶定位，评估了在指数生长培养中引发严格反应对抗生素生产的影响。actIII的表达在营养减少后出现，而在丝氨酸羟化酯治疗后无表达。虽然ppGpp是否需要触发抗生素的产生仍不明确，但单靠ppGpp合成似乎不足以启动S. coelicolor A3的次级代谢(2)。
The stringent response was elicited in the antibiotic producer Streptomyces coelicolor A3(2) either by amino acid depletion (nutritional shiftdown) or by the addition of serine hydroxamate; both led to increased levels of ppGpp and to a reduction in transcription from the four promoters of the rrnD rRNA gene set. Analysis of untreated batch cultures revealed elevated ppGpp levels at the end of exponential growth, preceding the onset of antibiotic production. The effect of provoking the stringent response on antibiotic production in exponentially growing cultures was assessed by S1 nuclease mapping of actIII, an early gene of the actinorhodin biosynthetic cluster. Expression of actIII occurred after nutritional shiftdown, but not after treatment with serine hydroxamate. Although the need for ppGpp in triggering antibiotic production remains equivocal, ppGpp synthesis alone does not appear to be sufficient to initiate secondary metabolism in S. coelicolor A3(2).