Molecular detection and prevalence of porcine caliciviruses in eastern China from 2008 to 2009
Molecular detection and prevalence of porcine caliciviruses in eastern China from 2008 to 2009
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DOI：
10.1007/s00705-009-0487-5
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发表时间：
2009-09
影响因子：
2.7
通讯作者：
Q. Shen;Wen Zhang;Shixing Yang;Yan Chen;H. Ning;T. Shan;Junfeng Liu;Zhibiao Yang;L. Cui
中科院分区：
文献类型：
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作者：
Q. Shen;Wen Zhang;Shixing Yang;Yan Chen;H. Ning;T. Shan;Junfeng Liu;Zhibiao Yang;L. Cui
近年来，在工业化国家和包括中国在内的发展中国家都有引起腹泻的杯状病毒的报道。猪杯状病毒与人痘病毒（SaV）和诺如病毒（NoV）密切相关，也已在猪中检测到，这引发了关于动物宿主和人畜共患病传播给人类的潜在风险的讨论。这项工作的目的是确定猪中SaV和NoV的频率和年龄分布，并表征中国东部流行的菌株。2008年4月至2009年3月，从中国东部收集了904份不同年龄猪的粪便样品，并使用逆转录-聚合酶链反应（RT-PCR）检测SaV和NoV。我们的结果表明，8份（0.9%）粪便样本对SaV呈阳性，2份（0.2%）对NoV呈阳性。RNA依赖的RNA聚合酶（RdRp）基因的部分序列的系统发育分析表明，所有的SaV株属于GIII SaVs，而两个NoV株属于GII NoV基因组。8株SaV可进一步分为两个簇，分别与荷兰株（AY 615804）和中国株（EU 599212）紧密聚在一起。这两株诺如病毒与GenBank中唯一的中国大陆诺如病毒株（DQ 369797）的核苷酸同源性为67.3-67.6%。此外，我们的研究结果表明，SaV感染更频繁地在0-1个月大的猪比在老年人。总之，本研究提供了证据，PoSaV和PoNoV存在于中国东部的猪。
Caliciviruses causing diarrhea have been reported in both industrial and developing countries, including China, in recent years. Porcine caliciviruses that are closely related to human sapoviruses (SaVs) and noroviruses (NoVs) have also been detected in swine, which has raised discussion about the animal reservoir and the potential risk for zoonotic transmission to humans. The objective of this work was to determine the frequency and age distribution of SaVs and NoVs in pigs and to characterize the strains prevalent in eastern China. A total of 904 stool samples from pigs of different ages were collected from eastern China from April 2008 to March 2009 and tested for both SaVs and NoVs using reverse transcription-polymerase chain reaction (RT–PCR). Our results indicate that 8 (0.9%) stool samples were positive for SaVs and 2 (0.2%) for NoVs. Phylogenetic analysis of partial sequences of the RNA-dependent RNA polymerase (RdRp) gene indicated that all of the SaV strains belonged to the GIII SaVs, while the two NoV strains belonged to the GII NoV genogroup. The 8 SaV strains were further divided into two clusters, which clustered closely with the Netherlands isolate (AY615804) and the Chinese strain (EU599212), respectively. The two NoV strains shared about 67.3–67.6% nucleotide homology with a human norovirus strain (DQ369797), the only NoV strain from mainland China available in GenBank. Moreover, our results suggest that SaV infections are more frequent in 0-1 month-old pigs than in older ones. In conclusion, the present study provides evidence that PoSaVs and PoNoVs exist in swine in eastern China.