Intestinal response to 1α,25,dihydroxycholecalciferol II. A timed study of the intracellular localization of calcium binding protein
Intestinal response to 1α,25,dihydroxycholecalciferol II. A timed study of the intracellular localization of calcium binding protein
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肠道对 1α,25,二羟基胆钙化醇 II 的反应钙结合蛋白细胞内定位的定时研究。
DOI：
10.1016/0304-4165(78)90249-0
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
T. Bucci
中科院分区：
文献类型：
--
作者：
R. Morrissey;R. Empson;D. Zolock;D. Bikle;T. Bucci
一日龄雏鸡饲喂缺钙饮食（0.43%Ca，0.3%P）3周，并在口服1 μ g 1 α，25-二羟基胆钙化醇和100 μ Ci氚化胸苷i/p后24小时内分批处死。固定十二指肠组织进行放射自显影和钙结合蛋白（CBP）的免疫组织学定位。CBP在给药后6 - 8 h首次见于绒毛基部肠吸收细胞的细胞核和细胞质中，至18 h遍及整个绒毛。胸苷标记显示CBP从绒毛基部到绒毛尖端的出现速度远快于细胞迁移速度。结果表明，绒毛基部的年轻细胞和绒毛尖端的成熟细胞都可以合成CBP，但年轻细胞的反应更快。CBP的相对细胞内浓度随时间降低，这表明该蛋白被分泌、被吸收细胞降解或在细胞中重新分布。
One-day-old chicks were fed a calcium-deficient diet (0.43% Ca, 0.3% P) for three weeks and were killed in groups of five at intervals up to 24 hours after an oral dose of 1 mu g 1 alpha ,25-dihydroxycholecalciferol and 100 mu Ci of tritiated thymidine i/p. Duodenal tissue was fixed for radioautography and immunohistological location of calcium-binding protein (CBP). CBP was first seen in the nuclei and cytoplasm of intestinal absorptive cells at the villus bases between 6 and 8 hours and throughout the villus by 18 hours after treatment. Thymidine labelling showed that the progression of CBP appearance from villus base to tip was much faster than cell migration rate. It was concluded that young cells at the base of the villus and more mature cells at the villus tip could synthesize CBP, but that young cells responded faster. The relative intracellular concentration of CBP decreased with time, which suggests that the protein is secreted, degraded by the absorptive cells, or is redistributed in the cells.