Magnetic particle motions within living cells. Measurement of cytoplasmic viscosity and motile activity.
Magnetic particle motions within living cells. Measurement of cytoplasmic viscosity and motile activity.
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DOI：
10.1016/s0006-3495(87)83244-7
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发表时间：
1987-10
影响因子：
3.4
通讯作者：
P. Valberg;H. Feldman
中科院分区：
文献类型：
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作者：
P. Valberg;H. Feldman
亚微米磁性颗粒，由细胞摄取，并通过它们产生的磁场监测，提供了一种替代光学显微镜探测活细胞质的运动和粘度，并可用于细胞在体外和体内。我们目前的方法制备肺巨噬细胞标记的磁性颗粒的磁研究。数据的解释涉及拟合实验残留场衰减曲线的非线性机制模型的细胞内粒子运动。模型参数是敏感的流动性和明显的细胞质粘度所经历的含颗粒的细胞器。我们目前的结果参数估计的细胞内颗粒的行为在控制细胞和后（a）可变磁化持续时间，（B）孵育细胞松弛素D，和（c）颗粒扭曲的外部领域。磁力分析表明，细胞质弹性，剂量依赖性的运动抑制细胞松弛素D，和剪切稀化的表观粘度。
Submicrometer magnetic particles, ingested by cells and monitored via the magnetic fields they generate, provide an alternative to optical microscopy for probing movement and viscosity of living cytoplasm, and can be used for cells both in vitro and in vivo. We present methods for preparing lung macrophages tagged with magnetic particles for magnetometric study. Interpretation of the data involves fitting experimental remanent-field decay curves to nonlinear mechanistic models of intracellular particle motion. The model parameters are sensitive to mobility and apparent cytoplasmic viscosity experienced by particle-containing organelles. We present results of parameter estimation for intracellular particle behavior both within control cells and after (a) variable magnetization duration, (b) incubation with cytochalasin D, and (c) particle twisting by external fields. Magnetometric analysis showed cytoplasmic elasticity, dose-dependent motion inhibition by cytochalasin D, and a shear-thinning apparent viscosity.