Enhanced expression of the apoptosis inducing ligand TRAIL in mononuclear cells after myocardial infarction
Enhanced expression of the apoptosis inducing ligand TRAIL in mononuclear cells after myocardial infarction
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DOI：
10.1536/jhj.44.833
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发表时间：
2003-11-01
期刊：
影响因子：
--
通讯作者：
Mizuguchi, J
中科院分区：
文献类型：
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作者：
Nakajima, H;Yanase, N;Mizuguchi, J
肿瘤坏死因子（TNF）家族蛋白包括TNF-α和Fas（CD 95）-配体，其与急性心肌梗死（AM 1）的发生有关。我们研究了AM 1患者是否表现出另一个TNF家族成员的上调。采用流式细胞术和RT-PCR方法检测26例AM 1急性期患者和16例健康对照者外周血单个核细胞（PBMC）中肿瘤坏死因子相关凋亡诱导配体（TRAIL）的表达。此外，还比较了来自AM 1急性期患者的PBMC上的TRAIL蛋白表达与来自相同患者7天后的PBMC上的TRAIL蛋白表达。应用流式细胞术检测急性期AM 1患者和正常对照组CD 4（+）、CD 8（+）、CD 14（+）和CD 19（+）细胞表面TRAIL蛋白的表达。最后，用荧光化学方法检测人心肌细胞中TRAIL受体（TRAILR）-1和TRAILR-2的表达。AMI急性期TRAIL蛋白表达明显高于对照组。AM 1急性期TRAIL蛋白表达明显高于7天后。AM 1急性期TRAILmRNA表达高于对照组。AML 1患者外周血CD 4+、CD 14+细胞的TRAIL蛋白表达明显高于对照组。结果表明，心肌细胞中存在TRAILR-1和TRAILR-2的表达，TRAIL油浸润的CD 4（+）和CD 14（+）细胞可能参与了AM 1诱导的心肌细胞凋亡。
Tumor necrosis factor (TNF) family proteins including TNF-alpha and Fas (CD95)-ligand have been implicated in the development of acute myocardial infarction (AM1). We studied whether AM1 patients displayed up-regulation of another TNF family member. TNF-related-apoptosis-inducing ligand (TRAIL), on peripheral blood mononuclear cells (PBMCs).We compared expression of TRAIL on PBMCs front 26 patients in the acute phase of AM1 with that on PBMCs from 16 healthy control subjects using flow cytometry and RT-PCR. In addition, expression of TRAIL protein on PBMCs from patients in the acute phase of AM1 was also compared with that from the same patients 7 days later. Furthermore, we compared the expression of TRAIL protein on CD4(+), CD8(+), CD14(+), and CD19(+) cells front patients in the acute phase of AM1 with that from control subjects using flow cytometry. Finally, expression of the TRAIL receptors (TRAILR)-1 and TRAILR-2 in human cardiomyocytes was examined immunohistochemically. Expression of TRAIL protein was significantly higher in the acute phase of AMI than in control subjects. Expression of TRAIL protein was significantly higher in the acute phase of AM1 than 7 days later. TRAIL mRNA expression in the acute phase of AM1 was higher than in control subjects. Expression of TRAIL protein oil CD4(+) and CD14(+) cells front AM1 patients was significantly higher than that from control subjects. Expression of TRAILR-1 and TRAILR-2 in human cardiomyocytes was confirmed immunohistochemically.TRAIL oil infiltrating CD4(+) and CD14(+) cells may be involved in (he induction of cardiomyocyte apoptosis after AM1.