Stabilization of a HemA-LacZ hybrid protein against proteolysis during carbon starvation in atp mutants of Salmonella typhimurium.
Stabilization of a HemA-LacZ hybrid protein against proteolysis during carbon starvation in atp mutants of Salmonella typhimurium.
复制标题
HemA-LacZ 杂合蛋白在鼠伤寒沙门氏菌 atp 突变体碳饥饿期间的蛋白水解稳定性。
DOI：
10.1128/jb.178.8.2462-2464.1996
复制
发表时间：
1996
影响因子：
3.2
通讯作者：
Elliott,T
中科院分区：
文献类型：
--
作者：
Archer,CD;Jin,J;Elliott,T
在碳饥饿后稳定HEMA-LacZ杂交蛋白的β-半乳糖苷酶活性的转座子插入被映射到鼠伤寒沙门氏菌的ATP操纵子。这种效应类似于在节能的I型NADH脱氢酶中存在缺陷的nuo突变体。其他几个基因的插入，包括高度多效性的突变体，如rpos，pola和hfq，用相同的表型筛选分离出来，但它们不影响HEMA-LacZ的β-半乳糖苷酶活性。所有这些突变体都间接地改变了指示板上许多不同融合菌株的菌落颜色。
Transposon insertions that stabilize the beta-galactosidase activity of a HemA-LacZ hybrid protein following carbon starvation were mapped to the atp operon of Salmonella typhimurium. This effect is similar to that seen with nuo mutants defective in the energy-conserving type I NADH dehydrogenase. Insertions in several other genes, including such highly pleiotropic mutants as rpoS, polA, and hfq, were isolated with the same phenotypic screen, but they do not affect the beta-galactosidase activity of HemA-LacZ. All of these mutants act indirectly to alter the colony color of many different fusion strains on indicator plates.